CD137信号对小鼠树突状细胞表面TLR4表达的调节  

Regulation of Toll-Like Receptor 4 Expression on Murine Dendritic Cells Mediated by CD137 Signal

作　　者：马倩茹[1] 张烽[1] 周桓[1] 於葛华[1] 张学光[1] 顾宗江[1] 

机构地区：[1]苏州大学医学生物技术研究所免疫学教研室,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2007年第1期11-13,40,共4页Suzhou University Journal of Medical Science

基　　金：江苏省临床免疫学重点实验室资金资助

摘　　要：目的探讨CD137(4-1BB)激发型单克隆抗体2A对小鼠未成熟树突状细胞株DC2.4表面TLR4表达的调节。方法以流式细胞术检测不同剂量2A、LPS,或LPS预刺激后再加入2A等因素作用下,DC2.4表面的TLR4表达情况。结果LPS下调TLR4表达,2A使DC2.4上TLR4表达上调,并拮抗LPS对TLR4的下调作用。LPS预处理后再加入2A,也拮抗LPS的下调作用。结论CD137信号可以上调DC2.4表面TLR4的表达。Objective To study the regulatory effect of agonist CD137 （4-1BB） monoclonal antibody 2A on TLR4 expression in a routine immature dendritic cell line DC2.4. Methods Flow cytometry was used to investigate the TLR4 expression on DC2.4 under following conditions： different doses of 2A, LPS or addition of 2A after prestimulation of DC2.4 by LPS. Results LPS could down-regulate TLR4 expression on DC2.4. On the contrary, 2A could up-regulate TLR4 expression on DC2.4. Moreover, 2A could inhibit the down-regulatory effect of LPS on TLR4 expression. When DC2.4 was stimulated by 2A after LPS prestimulation, 2A could still up-regulate TLR4 expression. Conclusion CD137 signal could up-regulate the expression of TLR4 on dendritic cell line DC2.4.

关 键 词：树突状细胞 TLR4 4-1BB DC2.4 

分 类 号：Q254[生物学—细胞生物学] R392.1[医药卫生—免疫学]