环氧合酶-2在原发性肝细胞癌中的表达及其与HCC临床病理特征之间的关系  

Expression of cyclooxygenase-2 in primary hepatocellular carcinoma and its relationship with clinical pathological features

作　　者：齐亚灵[1] 方艳秋[1] 段秀梅[1] 谭岩[1] 陈东[2] 

机构地区：[1]吉林大学第一医院中心实验室,长春130021 [2]广东医学院组织学与胚胎学教研室,湛江524023

出　　处：《中国免疫学杂志》2007年第11期989-992,共4页Chinese Journal of Immunology

基　　金：吉林省科技厅国际合作项目(20060417);(20050703-6);吉林省杰出青年资助基金(20050113)资助

摘　　要：目的:探讨COX-2在原发性肝细胞癌组织、癌旁组织及正常肝组织中表达情况及其与临床病理特征之间的关系。方法:采用流式细胞术(FCM)及免疫组化(SP)法对30例肝癌组织、20例癌旁组织及10例正常肝组织中的COX-2蛋白进行定量检测,并对临床资料进行回顾性分析。结果:(1)COX-2在HCC组织中的表达显著高于癌旁组织和正常肝组织,差异具有显著性(P<0.05)。(2)COX-2蛋白的表达与肿瘤的大小、部位、包膜、AFP、镜下门静脉癌栓的有无均无关(P>0.05)结论:COX-2在HCC组织中具有较高表达,其过表达是反应HCC生物学行为的有效指标,对HCC的发生发展具有一定作用有望成为HCC基因治疗的靶点,为肝癌的化学预防提供理论依据。Objective：To investigate the expression of cyclooxygenase-2 in primary hepatocellular carcinoma, in cancer surrounding.tissues and normal liver tissue and its relationship with clinical pathological features. Methods：The expression of COX-2 was detected in 30 cases of hepatocellular carcinoma, 20 cases of cancer surrounding tissue and 10 normal liver tissue by flow cytometry （FCM） and immunohistochemistry （SP）. The clinical data were analyzed retrospectively. Results： （ 1 ） The expression of COX-2 in the HCC tissue was significantly higher than in cancer surrounding tissues and normal liver tissue （P 〈0. 05）. （2） The expression of survivin was independence of tumor size, location, capsule, AFP, endoscopic portal vein thrombosis formation （P 〉0. 05）. Conclusion： The hyperexpression of COX-2 in tissue can reflex the biological behavior of HCC, and have very important role in the development of HCC. The specificness of COX-2 protein expression make it to be new target of tumor diagnosis and treatment. These results provide a theoretical basis for the chemoprevention of hepatoma.

关 键 词：COX-2 原发性肝细胞癌 免疫组织化学 流式细胞术 

分 类 号：R735.7[医药卫生—肿瘤]