戊四氮诱导癫痫大鼠空间学习记忆受损及海马突触后致密物95表达减少  

Impaired spatial learning and decreased PSD-95 expression in hippocampus of pentylenetetrazol-kindled seizure of rats

作　　者：王佩[1] 王维平[1] 王海祥[1] 刘瑞春[1] 王晓鹏[1] 范月辉[1] 胡华伟[1] 

机构地区：[1]河北医科大学第二医院神经内科,河北石家庄050000

出　　处：《基础医学与临床》2007年第11期1227-1230,共4页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C2007000852);河北省科技攻关项目(07276101D-21)

摘　　要：目的探讨戊四氮诱导癫痫对大鼠空间学习记忆功能的影响及海马突触后致密物95(PSD-95)的表达变化。方法戊四氮(PTZ)腹腔注射诱导慢性癫痫(CEP)模型,采用Morris水迷宫对两组大鼠进行行为学检测,运用免疫组织化学方法观察海马CA1、CA3区PSD-95的表达,反转录聚合酶链反应(RT-PCR)方法检测大鼠海马PSD-95mRNA的表达。结果癫痫组大鼠空间学习记忆能力受损;其海马CA1、CA3区PSD-95免疫阳性产物较对照组明显减少(P<0.05),同时伴有海马PSD-95mRNA表达下降(P<0.01)。结论戊四氮诱导癫痫大鼠空间学习记忆受损可能与海马神经元PSD-95的表达减少有关。Objective To explore the effect of pentylenetetrazol （PTZ）-kindled epilepsy on rats＇ learning, memory and the expression of postsynaptic density 95 （ PSD-95 ）. Methods Rats were given PTZ till being kindled successfully. Their behaviors were afterwards evaluated through Morris water maze and the expression of PSD-95 and PSD-95 mRNA in hippocampus was simultaneously determined by immunohistochemical and RT-PCR method respectively. Results The ability of the learning and memory of the epileptic rats were impaired. The immunoreactivity of PSD-95 decreased （P 〈 0. 05 ）and the expression level of PSD-95 mRNA in hippocampus decreased （ P 〈 0. 01 ） simultaneously. Conclusion The impairment of the ability of learning and memory of the epileptic rats might be related with the change of PSD-95 in hippocampal neurons.

关 键 词：诱导 癫痫 空间学习记忆 突触后致密物95 

分 类 号：R742.1[医药卫生—神经病学与精神病学]