罗格列酮抑制非酒精性脂肪性肝炎大鼠肝组织IKK-β的表达  

Inhibition of rosiglitazone on the expression of IKK-β in the livers of rats with nonalcoholic steatohepatitis

作　　者：赵彩彦[1] 周俊英[1] 刘雅静[1] 贾蓓[1] 王亚东[1] 崔俊峰[1] 

机构地区：[1]河北医科大学第三医院感染科,河北石家庄050051

出　　处：《基础医学与临床》2007年第11期1231-1235,共5页Basic and Clinical Medicine

基　　金：河北省自然科学基金(C200500780)

摘　　要：目的探讨罗格列酮对非酒精性脂肪性肝炎(NASH)大鼠肝组织中IκB激酶β(IKK-β)表达的影响及意义。方法健康雄性Wistar大鼠高脂饲料喂养16周建立NASH模型,应用不同剂量罗格列酮灌胃治疗,并继续高脂饲料喂养12周后空腹收集大鼠血清及肝组织,ELISA检测血清TNF-α水平,RT-PCR和免疫组化染色观察大鼠肝组织IKK-βmRNA、蛋白的表达。结果模型组大鼠血清TNF-α水平显著增高,肝组织IKK-βmRNA及蛋白表达明显增强(P<0.01),肝组织有不同程度的脂肪变性、炎症坏死和纤维化。罗格列酮治疗组上述变化显著缓解,且与罗格列酮剂量正相关。结论罗格列酮可以阻止NASH大鼠IKK-β的表达和TNF-α的生成,抑制肝脏炎症反应。Objective To investigate the effect and significance of rosiglitazone on the expression of inhibiting kappa B kinase beta （IKK-β） in the liver of rats with nonalcoholic steatohepatitis （NASH）. Methods The models of NASH in male Wistar rats were established by feeding with fat-rich diet for 16 weeks. They were treated with different doses of rosiglitazone by gastric lavage for 12 weeks, all rats were sacrificed at fasting. Then the serum and hepatic tissue were collected. The serum concentration of tumor necrosis factor alpha （TNF-α） was measured by enzyme linked immunosorbent assay （ELISA） ; The expression of IKK-β mRNA and protein was detected by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry staining analysis separately. Results In model group, serum TNF-α levels were elevated, and the expression of IKK-β mRNA and protein in livers were enhanced significantly （P 〈 0. 01 ）. The steatosis, inflammation and fibrosis were identified in the livers. All of these were improved in rats with NASH after treatment with different doses of rosiglitazone for 12 weeks. Conclusion Rosiglitazone may suppress the inflammation of liver in NASH by inhibiting the expression of IKK-β and production of TNF-α.

关 键 词：IκB激酶β 罗格列酮 非酒精性脂肪性肝炎 

分 类 号：R575.1[医药卫生—消化系统]