P53与妊娠滋养细胞肿瘤的增殖和凋亡  

Correlation of P53 expression with proliferation and apoptosis in gestational trophoblastic tumor

作　　者：胡建功[1] 敖亚洲[2] 谢朝辉[3] 宋立刚[2] 王宁[2] 董雪峰[3] 张祥宏[4] 

机构地区：[1]承德医学院附属医院病理科 [2]承德医学院附属医院肿瘤外科 [3]承德市肿瘤医院,河北承德067000 [4]河北医科大学病理研究室,河北石家庄050017

出　　处：《基础医学与临床》2007年第11期1277-1280,共4页Basic and Clinical Medicine

摘　　要：目的探讨P53在妊娠滋养细胞肿瘤(GTT)中的表达意义及其与GTT中细胞增殖、凋亡的关系。方法采用免疫组化SP法及核酸原位末端标记(TUNEL)法,检测妊娠滋养细胞肿瘤中P53和PCNA的表达率及细胞凋亡指数(AI)。结果在正常早期绒毛(NP)、完全性葡萄胎(CM)、侵袭性葡萄胎(IM)、绒癌(CCA)中,P53表达率(P53-Ⅰ)分别为4.12%、21.68%、39.61%和27.39%;PCNA表达率(PI)分别为10.40%、20.76%、53.60%和51.95%;凋亡指数(AI)分别为1.88%、2.59%、6.45%和1.26%;各组间比较均P<0.001。P53-Ⅰ和PI呈显著正相关(r=0.587,P<0.001),而与AI无明确的相关性。结论GTT的发生可能是由于机体的细胞增殖与凋亡调节系统失调所致;P53可能促进GTT中的细胞增殖活性,与GTT的发生、发展密切相关;未发现P53与GTT细胞凋亡的相关性。Objective To elucidate the features of the expression of P53, proliferation and apoptosis in gestational trophoblastic tumor（GTT）. Methods Ten cases of normal placenta, 10 cases of complete hydatidiform mole （CM）, 20 cases of invasive hydatidiform mole （IM） and 19 cases of choriocarcinoma （CCA） paraffin-embedded tissues were studied. P53 and PCNA were detected with immunohistochemistry, apoptosis was detected with termi- nal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL）. Results In NP, CM, IM and CCA, the P53 indexes （P53- Ⅰ ） were 4. 12%, 21.68%, 39.61% and 27.39% respectively with significant differences among them （P 〈0. 001 ）. The PCNA indexes （PI） were 10. 40% , 20. 76%, 53.60% and 51.95% respectively with significant differences among them （ P 〈 0. 001 ）. The apoptosis indexes （ AI ） in them were 1.88%, 2. 59%, 6. 45% and 1.26% respectively with significant differences （P 〈 0. 001 ）. Significantly positive correlation was found between P53-Ⅰ and PI（ r =0. 587 ,P 〈0. 001 ） , while there was no correlation between P53- Ⅰ and AI. Conclusion The process of GTT is associated with trophoblastic cell overproli feration. The increasing expression of muta-P53 may contribute to the cell proliferation in GTY and the progress of GTY. No correlation was found between P53-Ⅰ and AI.

关 键 词：妊娠滋养细胞肿瘤 P53 细胞增殖 凋亡 免疫组织化学 TUNEL 

分 类 号：R737.33[医药卫生—肿瘤]