E1A基因对裸鼠移植瘤生长抑制及其初步作用机制的实验研究  

The E1A Gene Inhibits Cervical Carcinoma Growth in Nude Mice Through Alterations in Caspase-3 and Survivin Expression

作　　者：申良方[1] 刘新菊[1] 王骋[2] 赵凯[2] 乐园[2] 申海菊[2] 

机构地区：[1]中南大学湘雅医院肿瘤科,长沙市410008 [2]中南大学卫生部纳米生物技术重点实验室

出　　处：《中国肿瘤临床》2007年第4期198-200,207,共4页Chinese Journal of Clinical Oncology

基　　金：国家863计划项目(编号:2002AA214011);湖南省卫生厅科学基金资助(编号:B2005-035)

摘　　要：目的:探讨E1A基因对人宫颈癌细胞裸鼠移植瘤生长的抑制作用及其相关作用机制。方法:通过裸鼠移植瘤实验,观察E1A基因对人宫颈癌细胞裸鼠移植瘤生长的的抑制作用。采用免疫组织化学染色检测E1A基因对肿瘤细胞凋亡及Survivin基因和Caspase-3基因表达的影响。结果:裸鼠移植瘤实验结果显示:Hela-E1A细胞形成的肿瘤较Hela和Hela-vect的出瘤时间晚,生长慢,瘤重小,抑瘤率分别为83.42%和84.74%。免疫组织化学染色显示:E1A基因组细胞凋亡数量较Hela和Hela-vect组明显增多,Caspase-3基因(凋亡促进因子)呈高表达,Survivin基因(调亡抑制因子)的表达明显降低。结论:E1A基因能够明显抑制人宫颈癌细胞裸鼠移植瘤的生长,该作用可能与E1A基因激活Caspase-3基因和降低Survivin基因的表达有关。Objective： To study the effects of suppression of the E1A gene in tumors in nude mice that arise after injection of human cervical carcinoma HeLa cells and the related mechanism of action. Methods： Immunohistochemical staining was used to detect the effect of the E1A gene （by PEI-Fe3O4NP mediation and transfection） on expression of survivin and caspase-3 and to observe apoptosis of the tumor cells. Results： The results showed that tumors arising from the Hela-E1A cells occurred much later compared to those arising from the Hela and Hela-vector cells. Hela-E1A cells also displayed slow growth and low tumor volume. The tumor control rates were 83.42% and 84.74%. Results of immunohistochemistry staining showed that the apoptosis rate of the Hela-E1A cells was significantly higher than that of the Hela and Hela-vector cells. Caspase-3, a pro-apoptotic gene, displayed increased expression, and survivin, an anti-apoptotic gene, showed decreased expression. Conclusions： The E1A gene can effectively inhibit cervical carcinoma tumor growth in nude mice and the mechanism may relate to the E1A-induced increased expression of the caspase-3 gene and the decreased expression of the survivin gene.

关 键 词：E1A基因 人宫颈癌细胞裸鼠 移植瘤 Smwivin基因 Caspase-3基因PEI-Fe3O4纳米粒 

分 类 号：R737.33[医药卫生—肿瘤]