聚-ε-赖氨酸发酵培养基的优化  

Optimization of Fermentation Medium for Poly-ε-lysine Production

作　　者：陈雄[1] 章莹[1] 袁金凤[1] 王实玉[1] 李冬生[1] 

机构地区：[1]湖北工业大学生物工程学院湖北省工业微生物重点实验室,湖北武汉430068

出　　处：《化学与生物工程》2007年第8期45-47,共3页Chemistry & Bioengineering

基　　金：湖北省教育厅青年资助项目(Q200514001)

摘　　要：采用正交实验对筛选的一株白色链霉菌Streptomyces albulus213发酵产聚-ε-赖氨酸(-εPL)的培养基成分进行了优化。结果表明,该菌株产-εPL的优化培养基为:葡萄糖3.0%,酵母粉0.5%,(NH4)2SO41.2%,KH2PO40.20%、K2HPO40.12%、MgSO4.7H2O 0.05%、FeSO4.7H2O 0.003%、ZnSO4.7H2O 0.008%。在优化培养基下,ε-PL摇瓶产量达到4.95 g.L-1,比优化前提高了27%。The fermentation medium for poly-ε-lysine from Streptomyces albulus 213 was optimized by orthogonal experiments. The optimal medium consisted of 3.0% glucose, 0. 5% yeast extract, 1.2% （NH4）2 SO4,0. 20% KH2PO4,0. 12% K2HPO4,0. 05% MgSO4·7H2O,0. 003% FeSO4 · 7H2O and 0. 008% ZnSO4 · 7H2O. The yield of ε-PL reached 4.95 g · L^-1 with optimal medium,which increased 27% compared to initial medium.

关 键 词：聚-ε-赖氨酸 白色链霉菌 培养基 优化 

分 类 号：TQ920[轻工技术与工程—发酵工程]