培养基因工程菌表达未酰化胸腺素α_1的研究  

Study on the Culture of Recombinant E.coli and Expression of Fusion Protein——Non-acyl Thymosin α_1

作　　者：程波[1] 段涛[2] 白凤武[3] 包永明[3] 丁洁[3] 安利佳[3] 

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430073 [2]广东药学院生命科学与生物制药学院,广东广州510006 [3]大连理工大学环境与生命科学学院,辽宁大连116012

出　　处：《化学与生物工程》2007年第8期48-51,共4页Chemistry & Bioengineering

摘　　要：通过摇瓶培养对含有Thymosin α1基因的重组pET32a质粒的工程菌BL21的生长和融合蛋白表达进行了研究。选择LB和TH培养基，考察了培养温度、摇瓶中培养基盛装量、葡萄糖添加量、诱导剂IPTG浓度、诱导时间等因素对工程菌生长和融合蛋白表达的影响。确定最适生长温度为35-37℃、最佳蛋白表达温度为30℃，对数生长中期诱导，诱导剂浓度为0.4-0.5 mmol·L^-1，诱导时间为4-6 h。其融合蛋白表达量占菌体蛋白总量的26.83%。The culture of recombinant Escherichia coli pET32a/thymosin/DHS-α was carried out in shake flasks. The effects of culture temperature, broth volume, addition amount of glucose,inducer IPTG concentration and induction time on the growth of strain and fusion protein expression were investigated in LB and TH culture medium. The results showed the temperature suitable for the growth of recombinant strain and fusion protein expression were 35-37℃ and 30℃ ,respectively. The induction should be made at the middle of exponential growth phase. Controlling the inducer concentration of 0. 4-0. 5 mmol · L^-1 and induction time of 4-6 h,a high heterogeneous protein expression level of 26. 83% based on the total protein was achieved.

关 键 词：未酰化胸腺素1α 重组细胞培养 融合蛋白表达 

分 类 号：TQ464.7[化学工程—制药化工]