DNA疫苗制备工艺研究  

Rearch on Manufacture Process of DNA Vaccine

作　　者：夏俊[1] 姜永萍[2] 简子健[1] 左青山[1] 王岳[2] 陈化兰[2] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室兽医生物技术国家重点实验室,哈尔滨150001

出　　处：《新疆农业大学学报》2007年第4期38-40,共3页Journal of Xinjiang Agricultural University

基　　金：国家重点基础研究规划"973"(G1999011902)

摘　　要：以禽流感DNA疫苗pCAOH5转化大肠杆菌JM109后,利用发酵罐进行发酵培养。将菌体悬浮裂解中和后,经澄清和浓缩处理,利用阴离子交换色谱对其进行纯化。结果表明实验室规模的技术路线完全能够为质粒DNA的工业化制备奠定技术基础。Our study was conducted on avian influenza DNA vaccine pCAOH5 which was transfermed into E. coli JM109 and fermented in fermentor. After bacteria was suspended,splitted and neutralized, plasmid DNA was purified by anion exchange chromatogram. The results revealed that the process in laboratory scale could absolutely lay the foundation for manufacture plasmid DNA in large scale.

关 键 词：DNA疫苗 禽流感 发酵 色谱 质粒 

分 类 号：S851.43[农业科学—预防兽医学]