大黄橐吾水溶性多糖的初步纯化及清除自由基活性研究  

Study on Preliminary Purification and Scavenging Free Radical Activity of Polysaccharide in Ligularia duciformis

作　　者：刘春兰[1] 周博[1] 杜宁[1] 王文蜀[1] 李转秀[1] 曾鸣[1] 张琳霞[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《中央民族大学学报（自然科学版）》2007年第4期341-345,共5页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：国家"985"课题-中央民族大学"985"重点科研项目(cun985-3-3)

摘　　要：为合理利用大黄橐吾提供一定依据,对大黄橐吾多糖进行提取分离纯化.大黄橐吾经热水浸提,乙醇沉淀得到大黄橐吾粗多糖.粗多糖经凯氏定氮法测得蛋白质含量为14.02%,苯酚-硫酸法测得多糖中糖含量为23.41%,硫酸-咔唑法测半乳糖醛酸含量为35.16%.粗多糖经Sevage法和酶法联合脱蛋白,得初步纯化的多糖.纯化多糖经红外吸收光谱分析,初步断定其官能团的存在及其所处状态.对大黄橐吾多糖进行清除自由基活性研究,结果表明大黄橐吾多糖对超氧阴离子自由基和羟自由基具有明显的清除效果,且初步纯化后多糖的清除效果比粗多糖好.The crude polysaccharide was extracted from Ligularia duciformis with hot water, and precipitated by ethanol. Then some identifiable properties of the crude polysaccharide were studied. The contents of protein was 14.02%, the contents of polysaccharide was 23.41% and the contents of GAL-A was 35.16%. The refined polysaccharide was prepared after protein being separated. Both crude and refined polysaccharide were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of refined polysaccharide was higher than that of crude polysaccharide.

关 键 词：大黄橐吾 多糖 提取 纯化 清除自由基作用 

分 类 号：Q539[生物学—生物化学]