食管鳞状细胞癌组织中EGF蛋白和mRNA的表达  

Expression of EGF protein and mRNA in esophageal squamous cell carcinoma tissue

作　　者：尹磊[1] 张功员[1] 刘键[1] 李晟磊[2] 赵秋民[2] 刘宗文[2] 陈奎生[2] 张钦宪[1] 

机构地区：[1]郑州大学基础医学院组织学与胚胎学教研室,郑州450001 [2]河南省肿瘤病理重点实验室,郑州450052

出　　处：《郑州大学学报（医学版）》2007年第6期1099-1101,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省教育厅自然科学研究基金资助项目2007310017;郑州市科技攻关基金资助项目064SGDS33218-28

摘　　要：目的:观察食管鳞状细胞癌组织中EGF的表达情况及其与食管鳞状细胞癌浸润、转移及发生发展的关系。方法:采用免疫组化SP法和原位杂交法分别检测62例正常食管黏膜组织、31例癌旁不典型增生组织及62例食管鳞状细胞癌组织中EGF蛋白和mRNA的表达。结果:正常食管黏膜组织、癌旁不典型增生组织及癌组织中EGF蛋白的阳性表达率依次增高,分别为27.4%、45.2%、69.4%,3组间比较差异有统计学意义(χ2=21.954,P<0.01);正常食管黏膜组织、癌旁不典型增生组织及癌组织中EGFmRNA的阳性表达率依次升高,分别为40.3%,48.3%,77.4%,3组间比较差异有统计学意义(χ2=18.494,P<0.05);食管鳞状细胞癌组织中EGF蛋白和mRNA的表达与癌组织的分化程度、浸润深度及有无淋巴结转移有关(P均<0.05)。结论:EGF过表达与食管鳞状细胞癌的浸润、转移及发生发展有关。EGF有望成为食管鳞状细胞癌早期诊断和判断预后的辅助指标。Aim：To investigate the expression of EGF in esophageal squamous cell carcinoma（ESCC） tissue and the relationship between it and infiltration, metastasis, occurrence and development of ESCC. Methods ： The expressions of EGF protein and mRNA were examined by immunohistochemical SP method and in situ hybridyzation in 62 cases of normal esophageal mucosa,31 cases of adjacent atypical hyperplasia and 62 cases of ESCC tissue, respectively. Results：In normal mucosa, adjacent dysplasia tissue and ESCC tissue, the expression rate of EGF protein was 27.4% , 45.2% , and 69.4% , respectively, and that of EGF mRNA was 40.3% , 48.3% , and 77.4% , respectively. There were significant differences among the 3 groups in the expression of EGF protein and mRNA （x^2 = 21. 954,x^2 = 18. 494, P 〈 0.01. The expression of EGF protein and mRNA in ESCC tissue were associated with the differentiation, invasion depth, and lymph node metastasis （P 〈 0.05 ）. Conclusion ： The over-expressions of EGF protein and mRNA in ESCC tissue are associated with invasion, metastasis and the occurrence and development of ESCC. EGF could become one of the indicators in the process of early diagnosis and prognosis of ESCC.

关 键 词：EGF 食管肿瘤 鳞状细胞癌 浸润 转移 

分 类 号：R735.1[医药卫生—肿瘤]