GFP^+-大鼠胚胎脊髓源神经干细胞的培养、分化和Neuregulin-1的表达  

Culture and Differentiation of Neural Stem Cells from Spinal Cord of GFP-Transgene Embryonic Rat and Expression of Neuregulin-1

作　　者：金进明[1] 潘力[1] 郑华[2] 汪洋[2] 孙燕[2] 寿雪飞[1] 

机构地区：[1]复旦大学附属华山医院神经外科,200040 [2]复旦大学上海医学院解剖胚胎学系,200032

出　　处：《中国临床神经科学》2007年第6期600-604,共5页Chinese Journal of Clinical Neurosciences

摘　　要：目的:从GFP+-大鼠胚胎脊髓分离和培养神经干细胞(NSC),观察NSC的分化功能和Neuregulin-1的表达。方法:从孕16d的GFP+-大鼠胚胎脊髓中分离、培养神经干细胞,用免疫组织化学方法观察神经球的Neuregulin-1表达及鉴定分化的细胞类型。结果:从大鼠胚胎脊髓能分离、培养出NSC。神经球能表达Neuregin-1和Nestin,并能进一步分化为神经元、星形胶质细胞和少突胶质细胞。结论:从GFP+-大鼠胚胎脊髓能分离和培养出NSC,该NSC具有分化为用于治疗中枢神经疾病的多种神经细胞的潜能。Aim ： To isolate and culture the neural stem cells （NSCs） from spinal cord of the GFP^＋- transgene embryonic rat, and to observe the multiple functions and Neuregulin-1 expression of NSCs. Methods ： NSCs were isolated and cultured from spinal cord of the GFP^＋-embryonic rat （E16d）, The Neuregulin-1 expression of neurospheres and identification of cellular types differentiated from NSCs were determined by the method of immuno-histochemistry. Results ： NSCs could be isolated and cultured from spinal cord of the GFP^＋-embryonic rat. The neurospheres could express Neuregulin-1 and Nestin, and it could further differentiate into neurons, astrocytes and olygodenrocytes. Conclusion： NSCs could be isolated and cultured from spinal cord of the GFP^＋-embryonic rat. The NSCs have multiple functions of which differentiate into various neurocytes used in the treatment of CNS diseases.

关 键 词：脊髓来源神经干细胞 培养 分化 NEUREGULIN-1 

分 类 号：Q189[生物学—神经生物学]