猪传染性胃肠炎病毒主要抗原基因S与M双基因共表达载体的构建  

Construction of vectors for expression of swine transmissible gastroenteritis virus S and M genes

作　　者：贺小英[1] 彭树英[1] 刘艳春 杨瑞锋[1] 张涌[1] 

机构地区：[1]西北农林科技大学物工程研究所,陕西杨凌712100 [2]内蒙古巴林右旗农牧业局,内蒙古赤峰025150

出　　处：《中国兽医杂志》2007年第11期6-9,共4页Chinese Journal of Veterinary Medicine

基　　金：国家"863"高科技资助项目(2001AA213081)

摘　　要：研究如何提高猪传染性胃肠炎病毒(TGEV)中主要抗原基因S与M对猪传染性胃肠炎的免疫调节作用,进一步探究基因疫苗的有效开发途径。方法:通过两种方法应用牛β-酪蛋白启动子构建TGEV的S和M融合基因表达载体以及构建IRES连接M和S双顺反子共表达核酸疫苗载体。结果成功构建好M和S融合基因表达载体及IRES连接的M和S双顺反子真核共表达载体,并为利用乳腺生物反应器生产可食性疫苗提供有效措施。This study was designed to study how to enhance the immune regulatory effects of TGEV S and M genes for further development of nucleic vaccine. With bovine β-casein promoter,a fusion S and M gene and co-expressive vector with the linkage of the internal ribosome entry site （IRES） were constructed. The recombinant eukaryotic expression vector was constructed successfully, which will provide efficient strategies for the production of edible vaccine by mammary gland bioreactor.

关 键 词：核酸疫苗 IRES 猪传染性胃肠炎病毒 共表达载体 

分 类 号：Q786[生物学—分子生物学]