This work was supported by the National Natural Science Found ation of China(32273000);the Qingdao Demonstration Project for People-benefit from Science and Techniques,China(23-2-8-xdny-14nsh and 24-2-8-xdny-4-nsh);the National Program of Undergraduate Innovation and Entrepreneurship,China(202310435039);the Open Project Fund of State Key Laboratory of Microbial Technology,China(M2023-03)。
Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)th...
supported by the China Mega-Project for Infectious Disease[2018ZX10102001,2018ZX10711001,2018ZX10734401 and 2018ZX10734404];The Project of the National Pathogen Resource Collection Center[NPRC-32];the SKLID Development Grant[2011SKLID104]
Objective This study aimed to identify internal ribosome entry sites(IRESs)in the open reading frame(ORF)of the Coxsackievirus B3(CVB3)genome.Methods The sequences of P1,P2,or P3 of the CVB3 genome or the truncated se...
part of the Dutch Research Council(NWO)Building Blocks of Lite programme(grant number 737.016.007).
Microalgae are used in food and feed,and they are considered a potential feedstock for sustainably pro-duced chemicals and biofuel.However,production of microalgal-derived chemicals is not yet economically feasible.Ge...
The need for co-ordinate,high-level,and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways.This work outlines the functionality and design of IRES-and ...