The nucleolus as a genomic safe harbor for strong gene expression in Nannochloropsis oceanica  

作　　者：Christian Südfeld Ana Pozo-Rodríguez Sara A.Manjavacas Díez RenéH.Wijffels Maria J.Barbosa Sarah D'Adamo 

机构地区：[1]Wageningen University,Bioprocess Engineering,PO Box 16,6700 AA Wageningen,Netherlands [2]Faculty of Biosciences and Aquaculture,Nord University,8049 Bodø,Norway

出　　处：《Molecular Plant》2022年第2期340-353,共14页分子植物（英文版）

基　　金：part of the Dutch Research Council(NWO)Building Blocks of Lite programme(grant number 737.016.007).

摘　　要：Microalgae are used in food and feed,and they are considered a potential feedstock for sustainably pro-duced chemicals and biofuel.However,production of microalgal-derived chemicals is not yet economically feasible.Genetic engineering could bridge the gap to industrial application and facilitate the production of novel products from microalgae.Here,we report the discovery of a novel gene expression system in the oleaginous microalga Nannochloropsis that exploits the highly efficient transcriptional activity of RNA po-lymerase I and an internal ribosome entry site for translation.We identified the nucleolus as a genomic safe harbor for Pol I transcription and used it to construct transformant strains with consistently strong trans-gene expression.The new expression system provides an outstanding tool for genetic and metabolic en-gineering of microalgae and thus will probably make substantial contributions to microalgal research.

关 键 词：NANNOCHLOROPSIS gene expression NUCLEOLUS RNA polymerase I IRES aIRES 

分 类 号：Q94[生物学—植物学]