Synthetic polycistronic sequences in eukaryotes  

作　　者：Xuekun Wang Mario Andrea Marchisio 

机构地区：[1]School of Pharmaceutical Science and Technology,Tianjin University,92 Weijin Road,300072,Tianjin,China

出　　处：《Synthetic and Systems Biotechnology》2021年第4期254-261,共8页合成和系统生物技术（英文）

摘　　要：The need for co-ordinate,high-level,and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways.This work outlines the functionality and design of IRES-and 2 A-peptide-based constructs by comparing different strategies for co-expression in polycistronic vectors.In particular,2 A sequences are small peptides,mostly derived from viral polyproteins,that mediate a ribosome-skipping event such that several,different,separate proteins can be generated from a single open reading frame.When applied to metabolic engineering and synthetic gene circuits,2 A peptides permit to achieve co-regulated and reliable expression of various genes in eukaryotic cells.

关 键 词：Synthetic biology 2A peptide IRES Polycistronic sequence 

分 类 号：Q943.2[生物学—植物学]