胰蛋白酶在猪慢性胰腺炎胰腺组织中的分布及免疫组化阳性标准  

Distribution of pancreatic trypsin in pigs with chronic pancreatitis,and a positive trypsin standard for immunohistochemical studies

作　　者：唐伟[1] 张小明[1] 赵建农[1] 舒健[1] 邵阳[2] 谢贤雍[3] 朱建均[3] 

机构地区：[1]川北医学院附属医院放射科,四川省南充市637000 [2]川北医学院附属医院生物医学工程,四川省南充市637000 [3]川北医学院附属医院病理科,四川南充市637000

出　　处：《世界华人消化杂志》2007年第29期3092-3096,共5页World Chinese Journal of Digestology

基　　金：教育部新世纪优秀人才支持计划;No.NCET-06-0820

摘　　要：目的:建立慢性胰腺炎(CP)胰蛋白酶免疫组化阳性判断标准,探讨胰蛋白酶在CP中的分布特征及与CP严重程度的关系.方法:取正常猪4例及不同程度CP猪14例的胰腺组织,用链菌素亲生物素-过氧化酶连接法(streptavidin peroxidase conjugated method,SP)免疫组化法检测胰蛋白酶在实验性CP胰腺组织中的分布特征并初步建立阳性判断标准,分析胰蛋白酶的阳性标准与CP严重程度的相关性.结果:胰蛋白酶在CP胰腺组织主要表现为腺泡细胞质内、间质及胰管内棕色或棕黄色染色,染色程度在轻度CP以强阳性(++)为主,中度CP以中等阳性(+)为主,重度CP以弱阳性(±)为主,3组染色程度之间差异有统计学意义(P<0.05).胰蛋白酶强阳性染色与慢性胰腺炎严重程度具有相关性(r=0.742).结论:胰蛋白酶原异常活化可见于腺泡细胞质内、间质及胰管内,建立了胰蛋白酶阳性判断标准.随着慢性胰腺炎实质纤维化,胰蛋白酶阳性率降低.AIM： To study a positive primary trypsin standard for the immunohistochemical study of pigs with chronic pancreatitis, the distribution of pancreatic trypsin in chronic pancreatitis, and correlation of the distribution of trypsin with severity of chronic pancreatitis. METHODS： Pancreatic tissues were from four normal pigs and 14 with chronic pancreatitis of varying severity. The distribution of trypsin and a positive standard for pancreatic trypsin in pigs with chronic pancreatitis were studied by streptavidin-peroxidase conjugated immunohistochemical methods. The correlation between the positive standard and the severity of chronic pancreatitis was analyzed. RESULTS： Trypsin was stained brown in the cytoplasm, extracellular mesenchyme and pancreatic duct. The degree of staining for trypsin was strong positive （＋＋） in mild chronic pancreatitis, moderately positive （＋） in moderate chronic pancreatitis, and weakly positive （±） in severe chronic pancreatitis. There were significant differences in the degree of trypsin staining among the three groups （P 〈 0.05）. The degree of staining was correlated with the severity of chronic pancreatitis （r = 0.742）. CONCLUSION： Trypsinogen is abnormally activated in the cytoplasm, extracellular mesenchyme and pancreatic duct in pigs with chronic pancreatitis. The positive standard for trypsin is reasonable. The level of positive staining for trypsin decreased following the increases in pancreatic fibrosis in chronic pancreatitis.

关 键 词：胰蛋白酶 慢性胰腺炎 纤维化 免疫组化 猪 胰腺组织 分布 

分 类 号：R576[医药卫生—消化系统]