人肺癌高转移动物模型的筛选及其细胞系的建立  

Screening for an animal model of human lung cancer with highly metastatic potential as well as establishment its correspording cell line

作　　者：姚明[1] 闫明霞[2] 刘蕾[2] 吴海燕[2] 荚德水[2] 孔韩卫[2] 张书霞[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]上海市肿瘤研究所实验病理研究室,上海200032

出　　处：《肿瘤》2007年第11期866-869,共4页Tumor

基　　金：上海市科学技术委员会登山行动计划项目(编号:064909001)

摘　　要：目的:建立人肺癌小鼠高转移模型及高转移细胞系,同时观察相关生物学特性,为肺癌转移机制和防治等研究提供有用的实验工具。方法:切除首代小鼠移植瘤,以延长动物生存时间而获得转移灶,从第二代起采用肺转移灶→皮下移植→肺转移灶→皮下移植的体内循环筛选方法建立NOD/SCID小鼠人肺癌细胞SPC-A-1皮下移植瘤高转移模型,并进行肿瘤的生长和转移情况、组织病理学观察,同时建立相应的高转移细胞系,进行各种相关生物学特性观察。结果:第一代移植瘤切除后转移率达66.7%,通过4代体内反复筛选建立了100%肺转移NOD/SCID小鼠模型及相应高转移细胞系,细胞生长行为和染色体分析等生物学特性观察表明该细胞系保持了原有的人肺腺癌的生物学特性。结论:应用体内筛选的方法成功建立了人肺癌皮下移植瘤高转移模型及高转移细胞系,为肺癌防治研究及抗转移实验治疗提供了理想的动物模型。Objective： To establish an animal model of human lung cancer in NOD/SCID mice and its cell line with highly metastatic potential and observe their correlated biological features in metastasis so as to provide an important useful tool for studying the metastatic mechanism, diagnosis, prevention and therapy of lung cancer. Methods：Human lung cancer cells SPC-A-1 were inoculated subcutaneously into NOD/SCID mice,metastatic tumor masses in lung were harvested from curative resection of subcutaneous tumor, and re-implanted into NOD/SCID mice for the second round of in vivo selection. Besides resection of subcutaneous tumor, the same manipulation was triplicately repeated. Eventually, we established a highly metastatic animal model of human lung cancer in NOD/SCID mice and its cell line with highly metastatic behavior. The cell growth behavior, metastasis, morphological characteristics of the implanted tumors were observed by light microscopy. The biological features of the highly metastatic cell line were investigated. Results：The lung metastasis rate of SPC-A-1 cells was 66.7% after curative resection of subcutaneous tumor in the first round in vivo screening. An animal model of human lung cancer with 100% lung metastasis and its cell line with unique metastatic characteristics were established by in vivo 4-round repeated screening. The cell growth behavior and karyotype analysis showed that SPC-A-1 cell line maintained the biological characteristics of primary human lung adenocarcinoma. Conclusion：A highly metastatic animal model of subcutaneous implanted human lung cancer and its cell line are successfully established by in vivo screening. Our study provides an ideal animal model for research of prevention of lung cancer and experimental therapy against metastasis.

关 键 词：肺肿瘤 模型 动物 肿瘤转移 免疫缺陷小鼠 体内筛选 

分 类 号：R734.2[医药卫生—肿瘤]