全自动细胞DNA定量分析在胸腔积液中的诊断价值  

Diagnostic application of quantitative DNA analysis for pleural fluid by automated image cytometry

作　　者：乔岩[1] 孙丽华[1] 马吉勇[1] 邹春芳[1] 王文晶[1] 

机构地区：[1]南京医科大学附属南京第一医院呼吸科

出　　处：《临床肿瘤学杂志》2007年第11期850-851,855,共3页Chinese Clinical Oncology

摘　　要：目的:探讨胸腔积液中细胞DNA的含量分析对良恶性胸腔积液的鉴别诊断价值。方法:46例胸腔积液患者,分为恶性和良性两组,采用全自动细胞DNA定量分析系统测定胸腔积液细胞DNA的含量,同时检测癌胚抗原(CEA)。结果:对于恶性胸腔积液,全自动细胞DNA分析诊断的敏感性为58.3%,特异性为100.0%,准确性78.3%,而CEA分别为54.2%、81.8%和67.4%。如两项指标中任何一项阳性,诊断的敏感性、特异性和准确性分别为87.5%,81.8%和84.8%;两项指标同时阳性,特异性可达100.0%。结论:全自动细胞DNA定量分析对胸腔积液的鉴别有重要的临床参考价值,联合胸腔积液的CEA检测,可以提高诊断的有效性。Objective：To investigate the value of quantitative DNA analysis in different diagnosing pleural fluid types. Methods ： Forty-six patients with pleural fluid were divided into malignant and innocent group. All patients were detected by quantitative DNA analysis with automated DNA image cytometry and CEA detection. Results：In malignant pleural fluid, the sensitivity of quantitative DNA analysis was 58.3%, specificity was 100% and accuracy was 78.3%. The sensitivity, specificity and accuracy of CEA were 54. 2%, 81.8% and 67.4%, respectively. The sensitivity, specificity and accuracy were 87. 5%, 81.8% and 84. 8% as one of the two markers was positive. When the two markers were both positive, the specificity was 100%. Conclusion：Quantitative DNA analysis with automated DNA image cytometry has good clinical application to diagnosis pleural fluid, especially when combined with CEA.

关 键 词：全自动DNA倍体分析 癌胚抗原 胸腔积液 

分 类 号：R730.43[医药卫生—肿瘤]