丹参酮诱导HL-60细胞分化及凋亡的流式细胞术分析  

Cytometric Analysis of Tanshinone Induced Differentiation and Apoptosis of HL-60 Cells

作　　者：黄韧敏[1] 袁淑兰[1] 宋毅[1] 黄光琦[1] 

机构地区：[1]华西医科大学肿瘤研究所,成都市610041

出　　处：《中国肿瘤临床》1997年第7期500-503,共4页Chinese Journal of Clinical Oncology

基　　金：卫生部科学基金

摘　　要：应用体外细胞培养及流式细胞仪技术，分析了丹参酮作用前后HL－60细胞的细胞周期分布、细胞增殖指数、p53、c－myc、c－fos及bc1－2基因产物的表达变化，并以全反式维甲酸为对照。结果表明：0．5μg．ml－1的丹参酮作用后，HL－60细胞趋向粒细胞系统分化，有部分细胞发生凋亡（11．8％），丹参酮通过阻止HL－60细胞进入S期而抑制其DNA合成，其作用的分子机制与c－fos基因的表达增高、c－myc基因及bc1－2基因的表达降低有关，诱导分化与细胞凋亡的关系尚有待进一步探讨。Using techniques of cell culture in vitro and flow eytometry,the cellcycle distribution, cell proliferation index and gene expression of c-myc,c-fos and bc1-2of HL-60 cells of leukemia patients treated by Tanshinone (Tan,a Chinese herbal extract) were analysed and compared with all-trans-retinoic acid (RA). The results showed that HL-60 cells tended to differentiate to granulocytes and that some cells(11-8% ) underwent apoptosis when treated with Tan at 0. 5μg/ml-1. Tan interferedwith cells entrance into S phase whereupon DNA synthesis of the cells was inhibited.The action of Tan at the molecular level was associated with high expression of c-fosgene and low expression of c-myc as well as bc1-2 gene. Much yet remains to be investigated about the relationship between the induced differentiation and apoptosis of thecells.

关 键 词：白血病 丹参酮 诱导分化 细胞凋亡 流式细胞术 

分 类 号：R733.7[医药卫生—肿瘤]