β-紫罗兰酮诱导SGC-7901细胞凋亡作用  

β-ionone induced apoptosis in SGC-7901 cells

作　　者：孙向荣[1] 刘家仁[1] 孙文广[1] 唐玄乐[1] 陈炳卿[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院环境卫生学教研室,哈尔滨150086

出　　处：《卫生研究》2007年第6期667-670,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助(No30200229);中国博士后基金资助(No2003033383)

摘　　要：目的探讨β-紫罗兰酮对胃腺癌SGC-7901细胞的凋亡作用及其可能机制。方法采用细胞生长曲线、核分裂、Hoechst33258荧光染色、透射电镜和WesternBlot方法,观察了β-紫罗兰酮对SGC-7901细胞的生长抑制作用和细胞凋亡诱导情况。结果β-紫罗兰酮对SGC-7901细胞生长和有丝分裂有明显地抑制作用,EC50值为(174.93±12.79)μmol/L;并且可诱导SGC-7901细胞产生凋亡,激活Caspase-3片断和降低ERK1/2蛋白的表达,呈剂量-反应关系。结论β-紫罗兰酮可诱导SGC-7901细胞产生凋亡,不仅作用凋亡途经,而且还影响MAPK途经。Objective In order to determine the inhibitory effects and apoptosis of β-ionone in human gastric carcinoma SGC-7901 cell line and its possible mechanism. Methods The assays of the curves of cell proliferation, cell mitosis, Hoechst 33258 dye stained, emission electronic microscope, and western blotting were employed. Results β- ionone could inhibit the growth and cell mitosis in SGC-7901 cells. The ECso value was （174.93±12.79）tanol/L. Morphology of apoptosis was observed by Hoechst 33258 dye stained and emission electronic microscope. The fragmentation of activated caspase-3 was induced and expression of Erkl/2 protein was decreased in SGC-7901 cells treated with different levels of β-ionone. Conclusion Thus β-ionone could induce apoptosis ih SGC-7901 cells and exactly mechanism needed further to he studied.

关 键 词：Β-紫罗兰酮 SGC-7901细胞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R730.1[医药卫生—临床医学]