大肠黏膜癌变过程中COX-2,Bcl-2及Bax表达的相关性  

Expression of COX-2,Bcl-2 and Bax in the Carcinogenesis of Colorectal Mucosa

作　　者：彭立 欧阳淼[2] 

机构地区：[1]湖南省澧县人民医院普外科,湖南澧县415500 [2]中南大学湘雅医院消化科,湖南长沙410008

出　　处：《医学临床研究》2007年第7期1140-1143,共4页Journal of Clinical Research

摘　　要：【目的】探讨COX-2,Bcl-2及Bax在大肠黏膜癌变过程中表达相关性。【方法】采用免疫组织化学法检测了正常结肠黏膜15例、慢性结肠炎20例、大肠癌旁组织50例、腺瘤30例及大肠癌组织50例中COX-2,Bcl-2及Bax蛋白的表达。分析COX-2、Bcl-2及Bax的表达与各种临床病理特征的关系及三者的相关性。【结果】COX-2在正常结肠黏膜,慢性结肠炎,大肠癌旁组织,腺瘤及大肠癌组织中的表达阳性率分别是6.7%,10.0%,40.0%,56.7%,90.0%,呈递增趋势。Bcl-2在5组中的表达阳性率分别为6.7%,10.0%,38.0%,46.7%,76.0%,呈递增趋势。Bax在5组中的表达阳性率分别为86.67%,75.0%,78.0%,76.7%,82.0%,五组之间阳性率比较均无显著性差异(P>0.05),但其阳性表达程度在正常组织,腺瘤样息肉,大肠癌三组之间呈递减趋势(P<0.05)。3者在大肠癌中的表达与患者性别、年龄、肿块大小无明显差异(P>0.05)。COX-2和Bcl-2在大肠癌组织中阳性反应呈显著正相关(P<0.05,r=0.653)。COX-2、Bcl-2及Bax在大肠癌高、中分化组中的表达率分别为100%,88.9%,85.2%,明显高于低分化组中的79.3%、60.9%、78.3%;在大肠癌Duke's A,B期中Bcl-2表达却显著高于C,D期。【结论】COX-2及Bcl-2在正常结肠黏膜、慢性结肠炎、大肠腺瘤、大肠癌组织中的表达均呈递增趋势,而Bax在正常结肠黏膜、大肠腺瘤、大肠癌组织中的阳性表达程度呈递减趋势,提示COX-2及Bcl-2表达上调和Bax表达下调与大肠黏膜癌变过程有关。COX-2在大肠癌中的表达与肿瘤分化程度有关,Bcl-2在大肠癌中的表达与肿瘤分化程度、Duke's分期有关。COX-2与Bcl-2的表达呈正相关。[Objective]To investigate the expression and the relationship of COX-2、Bcl2 and Bax in the Carcinogenesis of Colorectal Mucosa.[Methods]The expressions of COX-2、Bcl-2 and Bax in 15 normal colorectal mucous membrane（NCM）、20 chronic colonitis（CHC）、30 colorectal adenoma（CAA）、50 adjacent tissues of colorectal cancer（CC）and 50 colorectal cancer tissue（CAT） were detected by immunohistochemical staining.The correlations among COX-2、Bcl-2 and Bax expression as well as the clinical-pathological character were analyzed.[Results]The expression rate of COX-2 in NCM,CHC,CAT,CAA and CAT,was 6.67%,10%,40%,56.7%,90% respectively and manifested an ascending trend.The rates of Bcl-2 in the five groups were 6.67%,10%,38%,46.7%,76% respectively and also showed an ascending trend.The rate of Bax expression in the five groups was 86.67%,75%,78%,76.7%,82% respectively and there was no significant difference among the five groups.However,the positive degree in NCM,CAA and CC group exhibited a descending trend.The expressions of COX-2、Bcl-2 and Bax in human CC were not associated with sex,age and the size of tumor.A positive correlation was noted between expression of COX-2 and Bcl-2 in CC tissue.The rates of COX-2,Bcl-2 and Bax in highly and moderately differentiated CC were significantly higher（100%,88.9%,85.2%,vs 79.3%,60.9%,78.3%）.The expression of Bcl-2 was significantly higher in Duke＇s A,B stage than in Duke＇s C,D（90%,77.8%,vs 60%,57.2%）.[Conclusion]The expression of COX-2 and Bcl-2 increases while that of Bax decreases with the occurrence and development of CC,which indicates that COX-2 and Bcl-2 play important roles in the carcinogenesis and development of CC.At the same time,there is a positive relationship between COX-2 and Bcl-2.

关 键 词：肠粘膜 癌前状态 结肠肿瘤 前列腺素内过氧化物合酶 基因 BCL-2 原癌基因 

分 类 号：R735.35[医药卫生—肿瘤]