癌光啉(PSD-007)——激光照射体外净化白血病细胞的实验研究  

STUDIES ON PURGING LEUKEMIC CELLS BY PHOTOSEN SITIZER PSD 007 LASER PHOTORADIATION IN VITRO

作　　者：梁新文 齐振华[1] 曹萍[1] 唐果成[1] 李晓林[1] 肖健云[1] 赖金平[1] 

机构地区：[1]附属湘雅医院血液科

出　　处：《湖南医科大学学报》1997年第3期199-202,共4页Bulletin of Hunan Medical University

摘　　要：研究用铜蒸汽激光照射癌光啉（ＰＳＤ－００７）介导的光敏作用，对白血病细胞系（ＨＬ－６０）的杀伤敏感性表明：白血病细胞对光敏作用敏感，在ＰＳＤ－００７１０μｇ·ｍｌ－１，激光照射２Ｊ·ｃｍ－２的处理条件下，ＨＬ－６０被抑制９８％，而正常骨髓细胞存活４０±８％。将正常骨髓单个核细胞与ＨＬ－６０细胞按１００∶１比例混合后，ＨＬ－６０细胞仍能被选择性杀伤。电镜下见光敏作用后的ＨＬ－６０细胞生物膜结构明显受损。结果提示：ＰＳＤ－００７——激光照射能选择性杀伤白血病细胞。The sensitivity to photosensitization mediated by the hematoporphyrin photosensitizer PSD 007 of acute promyelocytic leukemic cell line (HL 60) was compared with normal human hemopoietic progenitor cells. The results showed that the leukemic cells were more sensitive. After being treated with 10μg·ml -1 PSD 007 followed by 2J·cm -2 copper vapor laser light irradiation, the clonogenic leukemic (HL 60) cells were reduced 98%, but the survival rate of human granulocyte macrophage colony forming units (CFU GM) was 40±8%. Mixing of normal human marrow cells with leukemic (HL 60) cells (ratio 100∶1) did not interfere with elimination of tumor cells. The ultrastructure changes of HL 60 cells treated by laser photoradiation was observed under the trans electronic microscope. The mitochondria, endoplasmic reticulum and cell membrane were involved. This means that the cell biomembrane is the main target to be attacked. It is considered that PSD 007 laser photoradiation therapy is efficient for killing leukemic cells.

关 键 词：白血病 光化学疗法 癌光啉 骨髓净化法 

分 类 号：R733.705[医药卫生—肿瘤]