Studies on the Refolding of Egg White Lysozyme Denatured by Urea Using ＂Phase Diagram＂ Method of Fluorescence  

作　　者：边六交 董发昕 梁长利 杨晓燕 刘莉 

机构地区：[1]National Engineering Research Center for Miniaturized Detection System/Shaanxi Lifegen Co. Ltd., College of Life Science, Northwest University, Xi'an, Shannxi 710069, China [2]Center of Laboratory, Department of Chemistry, Northwest University, Xi'an, Shannxi 710069, China

出　　处：《Chinese Journal of Chemistry》2007年第12期1896-1903,共8页中国化学（英文版）

基　　金：Project supported by the Natural Science Foundation of Shaanxi Province [No. 2001K 10-G3-（3）].

摘　　要：The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a ＂phase diagram＂ method of fluorescence. The result showed that in the refolding of the reduced egg white lysozyme, an intermediate state of an egg white lysozyme exists at the urea concentrations in a final renaturation solution being about 4.5 mol/L, their refolding follows a three-state model; while in the refolding of the non-reducing egg white lysozyme, two intermediate states exist at the urea concentrations being separately 4.0 and 2.5 mol/L, and their refolding follows a four-state model. Through the comparison between the unfolding and refolding of an egg white lysozyme in the urea solution, it was found that both of the refolding of reduced and non-reducing egg white lysozyme molecules was irreversible to their unfolding in the urea solution. Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a ＂phase diagram＂ method of fluorescence. The result showed that in the refolding of the reduced egg white lysozyme, an intermediate state of an egg white lysozyme exists at the urea concentrations in a final renaturation solution being about 4.5 mol/L, their refolding follows a three-state model; while in the refolding of the non-reducing egg white lysozyme, two intermediate states exist at the urea concentrations being separately 4.0 and 2.5 mol/L, and their refolding follows a four-state model. Through the comparison between the unfolding and refolding of an egg white lysozyme in the urea solution, it was found that both of the refolding of reduced and non-reducing egg white lysozyme molecules was irreversible to their unfolding in the urea solution. Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.

关 键 词：egg white lysozyme ＂phase diagram＂ method of fluorescence REFOLDING UREA 

分 类 号：O6[理学—化学]