云南高校图书馆联盟文献共享服务平台- REFOLDING

REFOLDING: 作品数：49被引量：65H指数：5; 导出分析报告; 相关领域：生物学更多>>; 相关作者：何凤田蔡绍皙唐蓓韩丽华安宇更多>>; 相关机构：重庆大学第三军医大学华中科技大学郑州大学更多>>; 相关期刊：《Journal of Biophysical Chemistry》《Acta Biochimica et Biophysica Sinica》《Journal of Life Sciences》《Tsinghua Science and Technology》更多>>; 相关基金：国家自然科学基金国家高技术研究发展计划广东省自然科学基金更多>>

Heterologous expression,refolding,and characterization of a calcium-independent phospholipase A1 from Streptomyces albidoflavus: 《Systems Microbiology and Biomanufacturing》2022年第3期487-497,共11页Shi Cheng Zitao Guo Chaojuan Liang Yi Shi Peng Geng Yu Xin Zhenghua Gu Liang Zhang; supported by the National Key Research&Developmental Program of China(2021YFC2100303,2018YFA0900300).; Phospholipase A1(PLA_(1))is a member of the hydrolase family with applications in various fields,especially in the food industry.A calcium-independent PLA_(1) from Streptomyces albidoflavus was expressed in E.coli BL2...; 关键词：Calcium-independent phospholipase A1 Heterologous expression Continuous refolding CHARACTERIZATION Escherichia coli

Spontaneous Unfolding and Refolding of Plantaricin α-Helix in Molecular Dynamics Simulation: 《Computational Molecular Bioscience》2019年第1期27-39,共13页Shaomin Yan Guang Wu; Antimicrobial peptides are promising therapeutic agents in view of increasing resistance to conventional antibiotics. Antimicrobial peptides usually fold in α-helical, β-sheet, and extended/random-coil structures. T...; 关键词：ALPHA-HELIX ANTIMICROBIAL Peptides Protein FOLDING Plantaricin A

On-column Refolding of Diphtheria Toxin Variant CRM197 by Different Metal-Chelating Affinity Chromatography Matrices: 《Journal of Chemistry and Chemical Engineering》2014年第12期1135-1141,共7页Alessandra Stefan Mattia Boiani Luca Longanesi Alejandro Hochkoeppler; We have previously shown that the diphtheria toxin variant CRM 197 （cross-reacting material 197） can be overexpressed in Escherichia coli at high levels, yielding insoluble aggregates, which were solubilized using u...; 关键词：REFOLDING chromatographic matrix cross-reacting material 197 metal-chelating affinity chromatography

Efficient Protein Refolding Using Surfactants at High Final Protein Concentration: 《Journal of Surface Engineered Materials and Advanced Technology》2014年第1期9-13,共5页Hidetaka Noritomi Yoshiyuki Kato Satoru Kato; The refolding of denatured hen egg white lysozyme (HEWL) was examined by surfactants at a high final refolded HEWL concentration (1 mg/mL). Hexadecyltrimethylammonium bromide (CTAB) and sucrose fatty acid monoester (D...; 关键词：Protein REFOLDING DILUTION Method Surfactant HEN Egg-White LYSOZYME

Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies: 《Bioresources and Bioprocessing》2014年第1期82-90,共9页Xiaojuan Wang Chaogang Bai Jian Zhang Aiyou Sun Xuedong Wang Dongzhi Wei; supported by a grant from the Ministry of Science and Technology(National Major Science and Technology Projects of China(No.2012ZX09304009)).; Background:The melanoma differentiation-associated gene-7(mda-7)/interleukin-24(IL-24)can induce apoptosis in a wide variety of tumor cell types,whereas it has no toxicity in normal cells.However,recombinant human mda...; 关键词：Escherichia coli EXPRESSION Inclusion body INTERLEUKIN-24 PURIFICATION REFOLDING

An engineered <i>Phlebia radiata</i>manganese peroxidase: expression, refolding, purification and preliminary characterization被引量：2: 《American Journal of Molecular Biology》2012年第4期359-370,共12页Usenobong F. Ufot Monday I. Akpanabiatu; Manganese peroxidases (MnPs) are interesting enzymes in protein engineering, aimed at maximizing industrial bioprocesses such as lignin degradation and biofuel production. cDNA of the secreted short-type of MnP from P...; 关键词：Phlebia RADIATA PEROXIDASE Manganese PEROXIDASE OVER-EXPRESSION Recombinant

Stability and Refolding of Prophenol Oxidase Protein with 2-Propanol in Drosophila melanogaster被引量：1: 《Journal of Life Sciences》2012年第8期952-956,共5页Eri Sato Kotomi Mita Nobuhiko Asada; Phenol oxidase in Drosophila melanogaster occurs as folded phase precursors designated as prophenol oxidase A1 and A3, and prophenol oxidase is activated with alcohol, especially 2-propanol, within a few minutes as un...; 关键词：STABILITY 2-propanol REFOLDING prophenol oxidase Drosophila melanogaster.

Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy: 《Journal of Biophysical Chemistry》2012年第4期317-323,共7页Soma Sekhar R. Chinnadayyala M. Santhosh Pranab Goswami; The reversible effect of microwave mediated denaturation of protein at low exposure time of 10 s has been demonstrated for the first time. The effect of microwave (2.45 GHz and 900 W) was confirmed in a homo-octameric...; 关键词：MICROWAVE Alcohol Oxidase PROTEIN UNFOLDING PROTEIN REFOLDING PICHIA PASTORIS PROTEIN structure

Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue被引量：1: 《Science China Chemistry》2012年第8期1657-1662,共6页BAE Sang-Woo CHANG Woo-Jin KOO Yoon-Mo HA Sung Ho; supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013308); The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies （IBs）. Thus, the additional in vitro protein refolding process is required to conve...; 关键词：protein refolding room temperature ionic liquid dilution refolding LYSOZYME HYDROPHOBICITY SULFUR

An efficient method for refolding the extraceUular portion of CD147 from the total bacterial lysate: 《Acta Biochimica et Biophysica Sinica》2011年第11期900-908,共9页Fei Song Xin Zhang Yifei Li Qiang Ru Xiaobai Ren Bin Xia Zhi-Nan Chen; This work was supported by the grants from the National Natural Science Foundation of China （30900234 F. Song）, and the Major State Basic Research Development Program of China （973 Program; 2009CB521700 ZN Chen and B. Xia）.; CD147 is a widely expressed transmembrane protein that mediates signal transduction, and it plays important roles in many physiological and pathological processes, such as tumor invasion and metastasis. The extracellu...; 关键词：CD 147 extracellular domain protein refolding isotope labeling nuclear magnetic resonance

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