云南高校图书馆联盟文献共享服务平台- PASTORIS

PASTORIS: 作品数：77被引量：224H指数：8; 导出分析报告; 相关领域：生物学更多>>; 相关作者：张爱联曹瑞兵罗进贤张添元陈溥言更多>>; 相关机构：中山大学南京农业大学华东理工大学中国热带农业科学院更多>>; 相关期刊：更多>>; 相关基金：国家高技术研究发展计划国家自然科学基金山东省自然科学基金中央高校基本科研业务费专项资金更多>>

Enhanced Palladium Adsorption by Immobilized Pichia pastoris: A Reusable Catalyst for Reduction of 4-Nitrophenol被引量：1: 《Journal of Materials Science and Chemical Engineering》2020年第4期91-97,共7页Liqin Lin Xiaolian Jing Shiping Chen; In this paper, the immobilized pellets were prepared by embedding Pichia pastoris (P. pastoris) with sodium alginate and used for the recovery of palladium ions in solution. The catalytic activity of immobilized Pd/P....; 关键词：PALLADIUM Recovery IMMOBILIZED Pichia PASTORIS

Cell Surface Display of Red-Grouper Nervous Necrosis Virus Capsid Protein on Pichia pastoris: 《Advances in Microbiology》2018年第11期830-845,共16页Uraiwan Intamaso Palatip Chutoam Witthaya Poomipak Nopadon Pirarat; Nervous necrosis virus (NNV), the etiological agent of viral nervous necrosis, has a high mortality rate of 100% in hatchery-reared larvae and juveniles. At present, there are still no effective vaccines available for...; 关键词：Nervous Necrosis Virus PROTEIN Engineering PROTEIN Expression P. PASTORIS Yeast Surface Display Technology

Study on Cellulase Gene Expressed in Pichia pastoris and Analyses of Its Biochemical Characters: 《Journal of Materials Science and Chemical Engineering》2018年第7期43-52,共10页Min Lu Juan Chang Ping Wang Qingqiang Yin Xiaowei Dang Tianzeng Gao Fushan Lu; Objectives: In order to increase cellulose degradation, cellulase was expressed in this study. Literature Review: Cellulose is the most abundant organic carbon source on Earth;its enzymatic hydrolysis will be very use...; 关键词：CELLULASE Gene Cloning and Expression BIOCHEMICAL Characters PICHIA PASTORIS

Effects of Deletions in Pichia pastoris RTGGenes on Phenotype and AOX1 Expression: 《Advances in Microbiology》2018年第5期439-450,共12页A. M. Rumyantsev G. A. Soloviev A. V. Slepchenkov E. V. Sambuk; Methylotrophic yeast Pichia pastoris is an object of modern biotechnology. Decisive understanding of gene regulation mechanisms is essential for successful protein production. In this study, we investigated the effect...; 关键词：PICHIA PASTORIS AOX1 Tor-Kinase Retrograde Regulation Pathway

Advances in engineering methylotrophic yeast for biosynthesis of valuable chemicals from methanol被引量：8: 《Chinese Chemical Letters》2018年第5期681-686,共6页Xingpeng Duan Jiaoqi Gao Yongjin J.Zhou; funded by the Young Investigator Grant from Dalian Institute of Chemicals Physics,Chinese Academy of Sciences(to Y.J.Zhou); Methylotrophic yeasts and bacteria, which can use methanol as carbon and energy source, have beenwildly used as microbial cell factories for biomanufacturing. Due to their robustness in industrial harshconditions, met...; 关键词：Microbial cell factory Methylotrophic yeast P. pastoris Methanol metabolism Biosynthetic pathways Metabolic engineering

葡萄糖碳源对组成型表达重组人C-反应蛋白的影响: 《中国医药导报》2017年第30期18-20,41,共4页李军明杨丽萍王臣玉赵琪郑清孙成铭; 山东省自然科学基金项目(ZR2015PH034); 目的探讨不同浓度的葡萄糖对Pichia pastoris组成型表达重组人C-反应蛋白(rhCRP)的影响并筛选出最优葡萄糖碳源。方法分别以浓度为0.5%、1.0%、1.5%、2.0%、2.5%、3.0%、3.5%(w/v)的葡萄糖作为碳源,利用毕赤酵母菌株组成型表达rhCRP。...; 关键词：C-反应蛋白葡萄糖碳源组成型表达 PICHIA PASTORIS

一株全基因合成的高温嗜碱甘露聚糖酶的酶学性质分析被引量：3: 《湖北农业科学》2017年第12期2356-2358,共3页汪斌张华山熊海容; 中央高校基本科研业务费专项资金项目(CZW15026;CZW15012); 根据来源于褐色高温单孢菌(Thermomonospora fusca)YX基因组序列,预测并合成了甘露聚糖酶ManB全基因序列(Accession No.KJ806638),与表达载体pPIC9K重组后,转化毕赤酵母(Pichia pastoris)GS115,筛选获得重组菌株ManB-GS115。其酶学性质...; 关键词：甘露聚糖酶毕赤酵母(Pichia pastoris) 基因合成酶学性质

两种半纤维素酶在毕赤酵母中的共表达: 《湖北农业科学》2017年第10期1971-1972,1979,共3页杨青张莉张华山熊海容; 中央高校基本科研业务费专项资金项目(CZW15026;CZW15012); 基于定向改造的木聚糖酶(DSB)和甘露聚糖酶(ManA),通过不同的整合位点和抗性标记实现这两种酶在同一宿主GS115中的整合与筛选,获得了共表达菌株GS115/DSB-ManA。其热稳定性检测结果表明,该共表达菌株产出的DSB与ManA均有较高的热稳定性...; 关键词：木聚糖酶(DSB) 甘露聚糖酶ManA 毕赤酵母(Pichia pastoris) 共表达

Survey of Intracellular Protein Extraction Methods from Pichia pastoris: 《World Journal of Engineering and Technology》2015年第4期1-6,共6页Yuli Wang Yuansen Hu Yang Lei Yangyong Lv Le Wang Hongwei Wei; The broken efficiency of cell wall and the release amount of Pichia pastoris intracellular protein under different cell breaking conditions were investigated in this paper. The results showed that broken efficiency us...; 关键词：PICHIA PASTORIS HIGH Temperature and ALKALI Treatment HIGH Pressure Homogeniza-Tion Cell Wall BREAKING Protein Extraction

Development of two methods for rapid screening of recombinant Pichia pastoris strains with high-level expression of β-mannanase: 《Journal of Central South University》2015年第11期4162-4167,共6页李古月郑甲周洪波; Project(CX2012B124)supported by the Graduate Degree Thesis Innovation Program of Hunan Province;China;Project(13JJ9002)supported by the Natural Science Foundation of Hunan Province;China;Project(2012XK4081)supported by the Key Science and Technology Plan of Hunan Provincial Science&Technology Department;China; The yeast Pichia pastoris(P. pastoris) has been used for the expression of heterologous proteins with the significant success. However, it is time-consuming to screen the high expression level of the recombinant P. pa...; 关键词：Β-MANNANASE PICHIA PASTORIS high-production STRAIN

PASTORIS