Microwave based reversible unfolding and refolding of alcohol oxidase protein probed by fluorescence and circular dichroism spectroscopy  

作　　者：Soma Sekhar R. Chinnadayyala M. Santhosh Pranab Goswami 

机构地区：[1]Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, India

出　　处：《Journal of Biophysical Chemistry》2012年第4期317-323,共7页生物物理化学（英文）

摘　　要：The reversible effect of microwave mediated denaturation of protein at low exposure time of 10 s has been demonstrated for the first time. The effect of microwave (2.45 GHz and 900 W) was confirmed in a homo-octameric alcohol oxidase in aqueous solution of pH 7.5. The unfolding events did not transverse through any intermediate states and no subunits of the protein were detached during the process. The refolding of the protein achieved at 4℃ for 24 h had regenerated the native enzyme. This reversible refolding approach excludes any chemical reagent and therefore established as simple technique for protein unfolding-folding studies.The reversible effect of microwave mediated denaturation of protein at low exposure time of 10 s has been demonstrated for the first time. The effect of microwave (2.45 GHz and 900 W) was confirmed in a homo-octameric alcohol oxidase in aqueous solution of pH 7.5. The unfolding events did not transverse through any intermediate states and no subunits of the protein were detached during the process. The refolding of the protein achieved at 4℃ for 24 h had regenerated the native enzyme. This reversible refolding approach excludes any chemical reagent and therefore established as simple technique for protein unfolding-folding studies.

关 键 词：MICROWAVE Alcohol Oxidase PROTEIN UNFOLDING PROTEIN REFOLDING PICHIA PASTORIS PROTEIN structure 

分 类 号：O6[理学—化学]