On-column Refolding of Diphtheria Toxin Variant CRM197 by Different Metal-Chelating Affinity Chromatography Matrices  

作　　者：Alessandra Stefan Mattia Boiani Luca Longanesi Alejandro Hochkoeppler 

机构地区：[1]Department of Pharmacy and Bioteehnology, University of Bologna, Bologna 4, 40136, Italy [2]CSGI (Center for Colloid and Surface Science), Department of Chemistry, University of Firenze, Sesto Fiorentino 3, 50019, ltaly

出　　处：《Journal of Chemistry and Chemical Engineering》2014年第12期1135-1141,共7页化学与化工（英文版）

摘　　要：We have previously shown that the diphtheria toxin variant CRM 197 （cross-reacting material 197） can be overexpressed in Escherichia coli at high levels, yielding insoluble aggregates, which were solubilized using urea. This study reports a comparison of three matrices suitable for the purification and refolding of recombinant CRM197 by metal-chelating affinity chromatography, Moreover, we show that refolded CRM197 features enzymatic activity.

关 键 词：REFOLDING chromatographic matrix cross-reacting material 197 metal-chelating affinity chromatography 

分 类 号：Q554.9[生物学—生物化学] Q786