NHE1反义基因磁性纳米微粒对SGC-7901胃癌细胞增殖和凋亡的影响  

Effect of NHE1 antisense gene magnetic nanoparticies on the proliferation and apoptosis of SGC-7901 cells

作　　者：刘海峰[1] 陈刚[2] 滕小春[2] 汪兴伟[2] 

机构地区：[1]武警总医院消化科,北京100039 [2]第三军医大学西南医院消化内科,重庆400038

出　　处：《现代肿瘤医学》2007年第12期1708-1710,共3页Journal of Modern Oncology

基　　金：武警部队科研基金资助项目(编号:wz200511)

摘　　要：目的:探讨NHE1反义基因磁性纳米微粒对SGC-7901胃癌细胞株增殖和凋亡的影响,探索胃癌基因治疗的新方法。方法:构建NHE1反义基因真核表达载体和NHE1反义基因磁性纳米微粒,将NHE1反义基因转染至SGC-7901胃癌细胞中,比较观察细胞转染前后生长曲线、细胞周期和细胞凋亡率的变化。结果:氧化铁磁性纳米颗粒成功地将NHE1反义基因转染至SGC-7901胃癌细胞中。NHE1反义基因能使SGC-7901胃癌细胞生长速度减慢、体外生长增殖能力降低,转染NHE1反义重组质粒的SGC-7901胃癌细胞增殖指数为34.73%,低于SGC-7901细胞的38.95%;NHE1反义基因使SGC-7901胃癌细胞凋亡率增加,转染NHE1反义重组质粒的SGC-7901胃癌细胞凋亡率为11.75%,显著高于SGC-7901胃癌细胞凋亡率(3.85%,P<0.01)。结论:NHE1反义基因磁性纳米微粒能抑制SGC-7901胃癌细胞增殖,诱导细胞凋亡,具有良好的治疗效果,有一定的临床应用前景。Objective ：To explore the effects of Na^＋ - H^＋ exchanger 1 （ NHE1 ） antisense gene magnetic nanoparti- cies on the proliferation and apoptosis of SGC - 7901 cells and its potentiel role in the genetherapy for gastric cancer. Methods： NHE1 antisense gene eukaryotic expression on vector pcDNA3.1 was constructed with recombinant DNA technique. The dextran coated iron oxide nanoparticles （DCIONP） was synthesized with deposition. NHE1 antisense gene eukaryotic expression plasmid was transfected into SGC - 7901 gastric cancer cells by DCIONP. We examined the changes of the proliferation index,growth curve and apoptotic rates. Results： NHE1 antisense gene magnetic nanoparticies were successfully constructed and transfected NHE - 1 antisense gene into SGC - 7901 cells. Compared with parent cells,the proliferation index in NHE- lantisense gene transfected SGC -7901 cells decreased from 38. 95% to 34.73% ,the apoptotic rates increased from 3.85% to 11.75% （ P 〈0.01）. The growth curve showed that the proliferative speed of NHE - 1 antisense gene transfected SGC - 7901 cells was markedly slower than that of SGC -7901 cells. Conclusion： NHE - 1 antisense gene magnetic nanoparticies can inhibit the proliferation and induce apoptosis of SGC - 7901 cells . This study provides a new strategy for the treatment of gastric cancer.

关 键 词：NHE1基因 反义基因治疗 氧化铁磁性纳米颗粒 胃癌 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]