6株NDV鄱阳株对肝癌细胞杀伤作用的初步研究  

Primary study on antitumor effect of six Newcastle disease virus strain Poyang on hepatocarcinoma cell

作　　者：杨建林[1] 宋德志[1] 唐深[1] 黄企光[1] 赖振屏[1] 樊晓晖[1] 

机构地区：[1]广西医科大学微生物免疫教研室,广西南宁530021

出　　处：《现代肿瘤医学》2007年第12期1738-1741,共4页Journal of Modern Oncology

摘　　要：目的:研究从江西鄱阳湖野鸭分离的6株NDV对肝癌细胞的杀伤效应,进一步筛选NDV溶瘤毒株。方法:用MTT法研究6株NDV鄱阳株对两株传代肝癌细胞株Novikoff和HepG-2及一株正常肝细胞株HL-7702的杀伤效应。结果:6株NDV鄱阳株对肝癌细胞株Novikoff和HepG-2有显著杀伤效应,而对人正常肝细胞HL-7702无明显影响;病毒对细胞的杀伤活性与病毒作用剂量和病毒作用时间成正比;病毒在肝癌细胞中有明显复制增殖现象;Novikoff肝癌细胞对NDV敏感性强于HepG-2肝癌细胞。结论:6株NDV鄱阳株对Novikoff及HepG-2肝癌细胞产生显著的杀伤作用,而对正常肝细胞HL-7702未见明显影响。Objective：Cytotoxicity assay of hepatocarcinoma cell treated with Poyang strains of Newcastle disease virus （NDV）. Methods： The cytotoxic effect of six NDV strains Poyang on the Novikoff ,HepG -2 and HL -7702 cell lines were investigated using MTT assay. Results： The apparently pathological changes were observed in Novikoff and HepG - 2 tumor cells, but not in HL - 7702 cells. The higher suppressed effect on Novikoff tumor cells than on HepG - 2 tumor cells in the NDV - strain Poyang. Conclusion ： NDV - strain Poyang can be a potential anticancer agent for the treatment of liver cancer.

关 键 词：NDV鄱阳株 肝癌细胞 抗肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]