CIK细胞联合白介素-2对鼻咽癌病人免疫功能及EB病毒指标的影响  

The effect to immune system and epstein- barr virus in the nasopharyngeal carcinoma patients by the adoptive immunotherapy of auto -cytokine induced killer cells

作　　者：秦海燕[1] 胡伟汉[2] 张晓实[2] 王其京[2] 徐韬[2] 梁颖[2] 

机构地区：[1]广州医学院第一附属医院,广东广州510060 [2]中山大学肿瘤防治中心,广东广州510060

出　　处：《现代肿瘤医学》2007年第12期1747-1749,共3页Journal of Modern Oncology

摘　　要：目的:细胞因子诱导杀伤细胞(cytokine-inducedkillercells,CIK细胞)是目前抗肿瘤过继细胞免疫治疗最为有效的方案。本研究通过治疗30例鼻咽癌病人,探讨CIK细胞联合IL-2对鼻咽癌病人免疫学功能及EB病毒指标的影响。方法:将符合条件的30例病人配对分为两组。治疗组病人接受CIK细胞过继细胞免疫治疗,每周一次,连续4周与对照组比较。免疫功能及EB病毒学指标分别通过测定外周血T淋巴细胞亚群,EB病毒VCA-IgA、EA-IgA、EDAb、DNA免疫荧光定量进行评估。结果:随访8～18个月,中位随访时间12个月。CIK治疗1个月后患者CD4+CD25+%明显下降,EB病毒VCA-IgA、EA-IgA、DNA免疫荧光定量下降显著。结论:CIK细胞可改善鼻咽癌病人的免疫功能,加速EB病毒抗体及DNA拷贝数下降。提示CIK治疗可消灭肿瘤微小残留灶及转移灶,可作为一种鼻咽癌的辅助治疗。Objective：It has been reported that adoptive immunotherapy with auto - Cytokine induced killer cells （CIK） was the most effective treatment in adoptive cellular immunotherapy. We will assess the effects of exvivo therapeutic , which derived from peripheral blood mononuelear cells （PBMCs） with the nasopharyngealeareinoma. Methads： Total of 30 nasopharyngeal carcinoma patients were enrolled, 15 patients received adoptive immunotherapy with auto- Cytokine induced killer cells. Compared with the other 15 patients （control group）. Immunologic responses were evaluated by T lymphoeytes subset changes respectively. EBV DNA , EDAb, VCA - IgA and EA - IgA were monitored in the period of treatment. Results： During follow -up of 6 - 18 months （median, 12 months ）, after adoptive immunotherapy with auto - Cytokine induced killer cells, the levels of CD4 ~ CD25 ~ % and EBV DNA , VCA - IgA and EA - IgA in experimental group were degraded significantly as compared with control group. Conclusion： Adoptive immunotherapy with auto - Cytokine induced killer ceils can degrad EBV DNA , VCA - IgA , EA - IgA, in- creased the immune function on nasopharyngeal carcinoma. It shows adoptive immunotherapy with CIK can eliminate micro residual and metastasis, and can be an adjunctive therapy.

关 键 词：鼻咽癌 细胞因子诱导杀伤细胞 过继细胞免疫治疗 

分 类 号：R739.63[医药卫生—肿瘤]