儿童NK/T细胞淋巴瘤的免疫表型及其与EB病毒的关系  

Immunophenotypic Features of NK/T Cell Lymphoma in Children and Its Association with Epstein-Barr Virus

作　　者：桂卫星[1] 明萌[1] 王大斌[1] 邹典定[2] 易建华[3] 张健[3] 

机构地区：[1]郧阳医学院附属太和医院儿科,湖北十堰442000 [2]武汉大学中南医院儿科,武汉430071 [3]郧阳医学院附属太和医院病理科,湖北十堰442000

出　　处：《实用儿科临床杂志》2007年第3期178-179,共2页Journal of Applied Clinical Pediatrics

基　　金：湖北省教育厅重点科研项目资助(B200624014)

摘　　要：目的探讨儿童NK/T细胞淋巴瘤的免疫表型特征及其与EB病毒(EBV)感染的关系。方法NK/T细胞淋巴瘤5例标本,采用免疫组织化学链霉素抗生物素-过氧化酶连接法(SP法)检测CD45RO、CD3ε、CD56、CD20、T细胞内抗原(TIA-1)、丝氨酸蛋白酶-粒酶B(Granzyme B)确定肿瘤细胞免疫表型及EBV潜伏膜蛋白(LMP-1),原位杂交技术检测EBV编码的RNA(EBER1/2)。结果NK/T细胞淋巴瘤5例CD45RO、CD3ε、TIA-1和Granzyme B全部阳性,CD56阳性2例,CD20全部阴性。EBER1/2阳性4例,LMP-1阳性3例。结论EBV感染与儿童NK/T细胞淋巴瘤关系密切,EBV感染在儿童NK/T细胞淋巴瘤的发生发展中可能起重要作用。Objective To study the immunophenotypic features of NK/T cell lymphoma in children and its association with Epstein-Barr virus （EBV） infection. Methods Five cases of children′s NK/T cell lymphoma were studied. CD45RO, CD3ε, CD56, CD20, TIA-1 and granzyme B were detected by immunohistochemistry staining for investigating immunophenotype. The expression of EBV-latent membrane protein （LMP-1） were detected by immunohistochemistry. EBV-encoded RNA （EBER1/2） were detected by in situ hybridization （ISH）. Results CD45RO, CD3ε, TIA-1 and granzyme B were positive in 5 cases, CD56 was positive in 2 cases, while CD20 negative in 5 cases.EBER1/2 positive in 4 cases and LMP-1 positive in 3 cases.Conclusions NK/T cell lymphoma in children is strong associated with EBV infection,and EBV infection may play an important role in the pathogenesis of children NK/T cell lymphoma.

关 键 词：NK/T细胞淋巴瘤 EB病毒 免疫组织化学 原位杂交 

分 类 号：R733.1[医药卫生—肿瘤]