肝细胞癌组织中VEGF、MMP-2和MVD的表达及其相关性研究  

Expressions of VEGF,MMP-2 and MVD in Hepetocellular Carcinoma Tissues and Their Significance

作　　者：吴飞翔[1] 曹骥[2] 赵荫农[1] 刘剑勇[1] 杨春[1] 李丽萍[1] 

机构地区：[1]广西医科大学附属肿瘤医院,广西南宁530021 [2]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《肿瘤学杂志》2007年第6期474-476,共3页Journal of Chinese Oncology

基　　金：广西青年科学基金资助项目(桂科青0447050)

摘　　要：[目的]研究肝细胞癌(HCC)组织中血管内皮生长因子(VEGF)及基质金属蛋白酶-2(MMP-2)蛋白的表达及其临床意义,并探讨其与血管生成的关系。[方法]采用免疫组化法检测60例肝癌组织及癌旁肝组织中VEGF及MMP-2的表达,用CD34标记免疫组化法检测微血管密度(MVD)。[结果]VEGF及MMP-2在癌组织中的阳性率分别为63.33%和60.00%,而在癌旁组织中的阳性率分别为33.33%和26.67%,癌组织与癌旁组织比较差异有显著性(P<0.01)。癌组织的MVD为54.92±8.55,癌旁组织的MVD为21.36±6.63,两者差异有显著性(P<0.01)。VEGF在人肝癌组织中的表达与术后复发、肝外转移、临床分期、门静脉癌栓、肿瘤直径相关。MMP-2在人肝癌组织中的表达与临床分期、门静脉癌栓、肝外转移及术后复发相关。在癌组织中MVD与VEGF及MMP-2的表达呈正相关,VEGF与MMP-2的表达亦呈正相关。[结论]HCC中VEGF及MMP-2的高表达与肿瘤血管形成有关,在HCC的发生、发展及术后复发过程中起重要作用。[Purpose] To study the expression and significance of the vascular endothelial growth factor （VEGF） and matrix metalloproteinase 2（MMP-2） in hepatocellular carcinoma （HCC） tissues, and to explore their correlation with angiogenesis of HCC. [Methods] Immunohistochemistry was used to determine the expression of VEGF, MMP-2 in 60 HCC tissues and cancer adjacent tissues, and microvessel density （MVD） was detected with labelled CD34. [Results] Positive rates of VEGF and MMP-2 in the HCC tissues （63.33% and 60.00%） were higher than those in adjacent tissues （33.33% and 26.67%,P〈0.01）. MVD in the HCC tissue was significantly higher that than in the adjacent tissue （54.92±8.55 vs. 21.36±6.63, P〈0.01 ）. The expression of VEGF in HCC tissue was significantly correlated with postoperative recurrence, extrahepatic metastasis, the clinical staging, portal vein tumor thrombus, and diameter of tumor. The expression of MMP-2 in HCC tissue was significantly correlated with clinical stage, portal vein tumor thrombus, extrahepatic metastasis and recurrence. MVD was positively correlated with expressions of VEGF and MMP-2, as well as VEGF and MMP-2. [Conclusion]The high expression of VEGF and MMP-2 in HCC tissues are correlated with the angiogenesis of HCC, which might play an important role in the carcinogenesis, progression and recurrence of HCC.

关 键 词：肝细胞癌 血管内皮生长因子 基质金属蛋白酶-2 微血管密度 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]