甲状腺癌淋巴结微转移的研究  

Study of lymph node micrometastasis in thyroid carcinoma

作　　者：李迅庚[1] 马宏岩[1] 金星[1] 

机构地区：[1]山东大学山东省立医院普通外科,山东济南250021

出　　处：《中国普通外科杂志》2007年第11期1073-1075,共3页China Journal of General Surgery

摘　　要：目的探讨MUCl检测在甲状腺癌淋巴结微转移的可靠性和敏感性。方法对488例甲状腺疾病手术患者于术前24h用1％美兰1．0—2．0mL注射于甲状腺结节或周围腺体，术中显示蓝染淋巴结，采用RT—PCR法测定临床蓝染淋巴结中的MUCl。结果蓝染淋巴结显示率甲状腺癌为93％，良性病例为0。研究组80个蓝染淋巴结中发现有MUClmRNA表达的为95％，与病理诊断率（86％）相比有提高（P〈0．05）；良性病变（阴性对照组）淋巴结均不存在MUClmRNA的表达；阳性对照组的癌转移性淋巴结均存在MUClmRNA的表达。结论MUCl较病理检查敏感，PCR产物点杂交进一步证实MUCl作为PCR标志物有较好的可靠性。Objective To evaluate the reliability and sensitivity of detection of thyroid carcinoma lymph node micrometastasis with MUC 1 . Methods In 488 cases of thyroid disease, 1% methylene blue 1.0 - 2.0mL was injected into the thyroid nodule or surrounding thyroid tissue 24hours before operation. The dye-stained lymph nodes were detected at operation and RT-PCR method was used to determine MCUI in the clinically blue-stained lymph nodes. Results The detection rate of the blue-stained lymph nodes in thyroid carcinoma was 93% , and in benign lesions was 0% . In the research group, 80 cases were found to have blue-stained lymph nodes, the detection rate was 95% and was higher than the pathologr diagnostic rate which was 86% （ P 〈 0.05 ）. In benign lesions （ negative control group ） , expression of MUC 1 mRNA was not found in any lymph node, while the expression of MUC 1 mRNA was present inall of the metastatic lymph nodes. Conclusions MUC1 is more sensitive than pathologic examination in detecting lymph node metastasis. PCR production spot hybridity further confirms that MUC 1, as a PCR marker, has satisfactory reliability.

关 键 词：甲状腺肿瘤/病理学 淋巴结微转移 MUCL 

分 类 号：R736.1[医药卫生—肿瘤]