裸鼠皮下前列腺癌模型的建立研究  

Establishment of nude mouse model of human prostate carcinoma by subcutaneous injection of cell line LNCaP

作　　者：郑彦博[1] 陶志华[1] 余凯远[2] 吴秀玲[3] 陈占国[1] 

机构地区：[1]温州医学院第一附属医院实验诊断中心,浙江温州325000 [2]温州医学院第二附属医院泌尿外科,浙江温州325000 [3]温州医学院第一附属医院病理科,浙江温州325000

出　　处：《温州医学院学报》2007年第6期540-542,共3页Journal of Wenzhou Medical College

摘　　要：目的:建立裸鼠皮下LNCaP前列腺癌模型,为前列腺癌动物实验研究提供工具。方法:以每只1×106LNCaP细胞数与基底膜基质混合注射入5只BALB/c裸鼠前肢腋窝皮下,于第2周至第12周分别检测裸鼠血浆tPSA含量,12w后处死裸鼠,取皮下肿瘤及相关器官做病理检查,监测肿瘤生长及转移情况。结果:80%(4/5)裸鼠于第8周后在前肢腋窝皮下长出肿瘤,肿瘤包膜完整,病理切片可见典型肿瘤细胞,但5只裸鼠均未发现肿瘤转移;5只裸鼠均可测得血浆tPSA,且血浆tPSA含量随肿瘤生长而升高,其中未长出实体瘤的裸鼠血浆tPSA含量亦随时间推移而升高。结论:成功地建立了裸鼠皮下LNCaP前列腺癌模型。该肿瘤模型能分泌PSA和表达DD3mRNA,能更有效地模拟人前列腺癌的生物学特性。Objective： To establish a nude mouse model by subcutaneous injection of cell line LNCaP as an useful research tool for human prostate carcinoma. Methods： 1 × 10^6 LNCaP cells mixed with Matrigel were subcutaneously injected into armpit of 5 BALB/c nude mice in each mouse. Plasma concentration of tPSA of each mouse was detected from the 2nd week to the 12th week. After the 12th week, the mice were executed. Histopathological examinations of subcutaneous tumors and relevant organs were performed to identify the oncogenesis and metastasis of prostate carcinoma. Results： Subcutaneous tumors in intact membranes had grown in 80% mice （4/5） after 8 weeks. There were lots of typical cancer cells in these tumors observed in pathological section. But there was no metastasis of prostate carcinoma found in all of 5 mice. In plasma of these 5 mice, tPSA can be detected. The test showed that plasma concentration of tPSA rose following upgrade of the size of tumor. And tPSA of the only mouse that has no tumor observed also rose following time advancement. Conclusion： The nude mouse model of human prostate carcinoma with subcutaneous injection of cell line LNCaP which can secrete PSA and express DD3mRNA is established. And it can simulate biological character of human prostate carcinoma more effectively.

关 键 词：LNCAP 前列腺癌 皮下模型 裸鼠 

分 类 号：R737.25[医药卫生—肿瘤]