HIF-1α在非霍奇金淋巴瘤中的表达及其与肿瘤细胞凋亡和增殖的关系  

Expression of hypoxia-inducible factor-1α and its relationship to apoptosis and proliferation in non-Hodgkin lymphoma

作　　者：蒋洁[1] 黎岳南[2] 牛海艳[2] 谈顺[2] 吴文川[2] 黎日海[2] 

机构地区：[1]海南医学院科研部,海南海口571101 [2]海南医学院病理学教研室,海南海口571101

出　　处：《海南医学院学报》2007年第6期523-526,共4页Journal of Hainan Medical University

基　　金：海南省教育厅课题资助(编号:Hjsk200324)

摘　　要：目的:研究HIF-1α在非霍奇金淋巴瘤中的表达及其与肿瘤细胞凋亡和增殖的关系。方法:利用免疫组织化学检测了77例胃MALT淋巴瘤bcl-2及fas蛋白的表达。结果:在NHL中,HIF-1α中呈高表达,阳性率为89.6%,高度恶性组的阳性表达强度明显高于低度恶性组(P<0.01),但B细胞型与T细胞型之间比较无明显差异(P>0.05);Caspase-3阳性表达率为(79.2%),低度恶性组的阳性信号强度明显低于高度恶性组(P<0.05);PCNA的阳性率94.8%,高度恶性组的阳性表达强度明显高于低度恶性组(P<0.01);在B细胞型和T细胞型NHL中,低度恶性和高度恶性组中HIF-1α与Caspase-3及PC-NA的阳性信号强度均呈明显的正相关关系。结论:在NHL的发生发展过程中,HIF-1α通过调控肿瘤细胞的高增殖和高凋亡发挥作用,并在其恶性转化中可能起着重要作用。因此,检测HIF-1α有可能作为判断该肿瘤的生物学行为和临床预后的指标之一。Objective： To study the expression of hypoxia-inducible factor-1α（ HIF-1α ）in non-Hodgkin lymphoma （NHL） and its relationship to apoptosis and proliferation of the tumor cell. Methods： Immunohisto- chemistry was used to detect expressions of both bcl-2 and fas proteins in 77 cases of gastric lymphoma of mucosaassociated lymphoid tissue（MALT）. Results： The positive expression rate of HIF-1α was 89.6% in 77 NHL, The positive rate and intensity of HIF-1α in high-grade NHL was significantly higher than that low-grade （ P〈 0.01 ）, but there was no significant difference between B cell type and T cell type of NHL（ P〉 0.05）. The positive expression rate of Caspase-3 was 79.2% in all NHL, low-grade NHL was significantly lower than that in high-grade（ P〈 0.05）, and the positive expression rate of PCNA was 94.8% in all NHL, the positive rate and intensity of PCNA in high-grade NHL was significantly higher than that in low-grade （P〈0.01）. There was significant positive correlation between the expression intensity of HIF-1α and Caspase-3. Conclusion： The data suggest that HIF-1α plays an important role in NHL genesis and development by increasing apoptosis and proliferation of the tumor cells. To detect the expression of HIF-1α can be one of the makers of biological behavior and clinical prognosis in NHL.

关 键 词：淋巴瘤 非霍奇金 转录因子 基因表达 细胞凋亡 免疫组织化学 

分 类 号：R733[医药卫生—肿瘤]