8 Hz、90 dB/130 dB次声作用对大鼠海马NMDAR1表达的影响  

Effects of 8 Hz,90 dB/130 dB infrasound on expression of NMDAR1 in hippocampus of rat

作　　者：刘朝晖[1] 陈景藻[2] 刘静[2] 邱建勇[2] 鲁睿[1] 袁晓朝[1] 

机构地区：[1]第四军医大学唐都医院理疗科,西安710038 [2]第四军医大学西京医院理疗科

出　　处：《中华物理医学与康复杂志》2007年第11期728-732,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：全军医学科学技术研究"十五"计划指令性课题(01L071)

摘　　要：目的探讨8 Hz、90 dB/130 dB不同声压级次声作用对海马细胞N-甲基-D-天门冬氨酸受体1(NMDAR1)表达的影响。方法将雄性SD大鼠88只随机分为11组,即对照组,90 dB次声作用1,7,14,21,28 d组,130 dB次声作用1,7,14,21,28 d组,每组8只。应用免疫组织化学方法,分别观察8 Hz、90 dB/ 130 dB次声作用不同时间点大鼠海马CA1、CA3和DG区细胞内NMDAR1表达。结果8 Hz、90 dB次声作用对海马各区NMDAR1表达的影响呈现下降→回升→显著增高→回落→恢复的变化规律;在所观察各组中,14 d组海马各区NMDAR1表达至峰值。8 Hz、130 dB次声作用对海马各区NMDAR1表达的影响与90 dB次声作用效应相反,呈现升高→下降→显著下降→回升→恢复的变化规律;在所观察各组中,14 d组海马各区NMDAR1表达降至最低点。结论8 Hz、90 dB/130 dB次声作用后,大鼠海马细胞NMDAR1均有较为敏感的反应和可逆性变化。海马不同区域对不同声压级次声作用的敏感性具有一定差异性。这些变化可能影响海马学习记忆功能。Objective To study the effect of infrasound on the changes of expression of NMDAR1 in hippoeampal cells. Methods Eighty-eight male Sprague-Dawley rats were randomized into eleven groups： control group, 90 dB/l d,7 d,14 d,21 d and 28 d infrasound exposed groups;130 dB/1 d,7 d,14 d,21 d and 28 d infrasound exposed groups. All the animals in the test groups were put in an infrasound field with 8 Hz, 90 dB or 130 dB for 2 hours daily. Immunohistoehemistry methods were used to detect the changes of intraeellular expression of NMDAR1 in hippoeampal cells. Methods The expression of NMDAR1 in hippoeampus after the rats were exposed to infrasound of 8 Hz, 90 dB SPL showed a procedure from reducing on the 1st day to rising on the 7th and peaked on the 14th day, then to descending on 21st day and returning to the standard level on the 28th day. Exposure to infrasound of 8 Hz, 130 dB SPL induced opposite effects on the expression of NMDAR1 compared with 90 dB SPL, which showed a process of increasing, descending, reaching to the lowest, then ascending and returning to the normal. The lowest expression of NMDAR1 occurred on the 14th day in every observed hippoeampal area. Conclusion 8 Hz, 90 dB/130 dB infrasound induced certain reversible reaction in the expression of NMDAR1 of hippoeampal cells in rats, which may disturb their learning and memory function.

关 键 词：次声 N-甲基-D-天门冬氨酸受体1 学习记忆 钙离子 海马 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]