含NGR的多肽与肿瘤细胞作用的NMR研究  

NGR Peptide Binding to Human Breast Cancer Cell Studied by NMR Spectroscopy

作　　者：吴昌琳[1] 叶海峰[2] 黄静[2] 吴自荣[2] 

机构地区：[1]上海市功能磁共振成像重点实验室华东师范大学物理系 [2]华东师范大学生命科学学院,上海200062

出　　处：《波谱学杂志》2007年第4期475-479,共5页Chinese Journal of Magnetic Resonance

基　　金：上海市功能磁共振成像重点实验室(华东师范大学)开放课题基金资助(FMRI061208)

摘　　要：NGR为肿瘤血管特异导向肽,将其与功能肽KV7连接构建成一导向功能多肽NGR-KV7;为研究该多肽与人肿瘤乳癌细胞的相互作用,应用核磁共振技术来探讨该多肽与细胞的作用位点和亲和性;通过1H NMR和T2弛豫谱的分析,推断该多肽分子与人乳癌细胞结合紧密,亲和程度高.NRG is a specific peptide with targeting binding affinity to tumor blood vessel. In this study, tumor-targeting functional peptides were constructed by connecting NGR peptide with functional peptides. To study the interaction between the constructed peptides with human breast cancer cell, binding epitope and affinity were measured with NMR spectroscopy. The spectroscopic and relaxation data showed that these peptides can bind to human breast cancer cell with high affinity.

关 键 词：NMR 相互作用 T2弛豫谱 NGR 

分 类 号：R730.23[医药卫生—肿瘤]