从土壤中筛选产漆酶微生物菌株的研究  

Screening of Laccse-producing Microbial Stains from Soil

作　　者：王剑锋[1] 刘建玲[1] 王璋[1] 

机构地区：[1]中国食品发酵工业研究院

出　　处：《食品与发酵工业》2007年第10期35-39,共5页Food and Fermentation Industries

摘　　要：根据漆酶催化特定底物氧化还原反应结果的特征,设计了1个以采用愈创木酚和α-萘酚作为产酶菌株限制性驯化因素并结合木质素作为产酶诱导强化剂的筛选摸版,在对保藏和外购的常见微生物菌株进行产酶性能分析,发现不能产酶或活性很低后,从土壤中分离得到40株产酶微生物。在经过进行选择性初筛分析和摇瓶发酵测定酶活的复筛试验的一系列反复筛选驯化比较,得到了3株分泌酶活量较高的丝状真菌W—E2、W—H1和W—12菌株,其胞外漆酶活力分别为155.4 U/mL、172.3 U/mL和188.2 U/mL,显示出了十分有效的开发潜力。According to the properties of the oxy-reduction catalyzed by laccase, an effective screening method to isolate laccase-producing microorganisms from soil was designed by using guaiacol and a-naphthol as the restrictive selection factors with the addition of lignin as the inducer to enhance the enzyme production. After the analysis for the enzyme productivity of the general microorganisms preserved in our laboratories or purchased from other institutions was carried out and there were no expected laccase activities among these strains, this method was successfully used to isolate out 40 potent laccase-producing microbial strains from soil samples. Three high efficient filamentous fungal strains W-E2, W-H1 and W-I2 were further selected after a serial comparative sieving through the primary screening with this method and secondary isolation by determining the actual laccase activity, and their extracellular laccase activities were 155.4 U/mL, 172.3 U/mL and 188.2 U/mL, indicating a very significant developing potential for industrial production.

关 键 词：漆酶 土壤分离 菌种选育 丝状真菌 细菌 

分 类 号：Q93[生物学—微生物学]