三七总皂甙对抗大鼠免疫性肝纤维化的实验研究  

Effects of total saponins of panax notoginseng against liver fibrosis in rats

作　　者：张桂灵[1] 石小枫[2] 冉长清[3] 徐曼[4] 朱照静[4] 

机构地区：[1]浙江省余姚市人民医院感染科,余姚315400 [2]重庆医科大学附属第二医院感染科,教育部感染性疾病分子生物学重点实验室,重庆400010 [3]重庆市中药研究院中药药理室,重庆400065 [4]重庆医科大学基础医学院药学教研室,重庆400016

出　　处：《第三军医大学学报》2007年第23期2212-2214,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30671867);重庆市自然科学基金(2004)~~

摘　　要：目的进一步观察三七总皂甙抗大鼠免疫性肝纤维化的作用。方法用牛血清白蛋白等复合因素致大鼠肝纤维化,同时给予三七总皂甙60mg/kg和30mg/kg皮下注射治疗,共6周。分离血清,检测肝功能;分离肝组织,作病理学纤维化程度分级和免疫组化观察Ⅰ、Ⅲ型胶原及TGF-β1的合成表达。结果三七总皂甙能降低血清转氨酶,增加肝脏白蛋白的合成,减轻肝纤维化程度,抑制免疫性肝纤维化大鼠Ⅰ、Ⅲ型胶原及TGF-β1的合成表达。结论三七总皂甙具有抗肝纤维化作用。Objective To investigate the effect of total saponins of panax notoginseng （PNS） on the production of collagen Ⅰ , Ⅲ and TGF-[3, in rats with experimental fibrosis. Methods Experimental fibrosis model was copied by intracutaneous injection of BSA and rats feeding on diet rich in lipid. From the 1 st clay after BSA injection, SPN （60 or 30 mg/kg） or Colchicine were given intracutaneously once a clay for 42 days. All animals were sacrificed on the 43rd clay. Their hepatic function was evaluated by determining the levels of ALT, AST, ALB in serum. The progression of hepatic fibrosis was confirmed by pathological analysis. Then type collagen Ⅰ , Ⅲ and TGF-β1 were observed with immunohistochemical method. Results The BSA injection significantly elevated the levels of ALT and AST, while SPN treatment significantly clown-regulated them. But the level of ALB was opposite to that of ALT or AST in SPN treatment group. The positive staining of the collagen I , Ill and TGF-β1 was stronger in hepatic fibrosis model group than in SPN treatment group. The contents of the collagen were identical to the immunohistochemical results. Conclusion SPN has the protective effect against liver fibrosis.

关 键 词：三七总皂甙 肝纤维化 Ⅰ Ⅲ型胶原 TGF—β1 

分 类 号：R-33[医药卫生] R285