大肠埃希菌Mn-SOD在乳酸乳球菌中的表达  

Expression of the manganese superoxide dismutase gene in Lactococcus lactis

作　　者：刘方久[1] 张德纯[1] 蒲荣[1] 

机构地区：[1]重庆医科大学临床检验诊断学省部共建教育部重点实验室重庆医科大学病原生物学教研室,重庆400016

出　　处：《中国微生态学杂志》2007年第6期481-482,485,共3页Chinese Journal of Microecology

基　　金：重庆市科委项目;编号[2002]62号

摘　　要：本文根据GenBank中报道的大肠埃希菌MG1655全基因组DNA序列中SOD的编码基因序列设计引物,PCR扩增大肠埃希菌锰超氧化物歧化酶(Mn-SOD)基因,并将其克隆入原核高效表达质粒载体pBV220中构建重组质粒pBV220-sod,并将其电转入乳酸乳球菌MG1363中获得了成功表达,为SOD发酵奶的研制奠定了基础。According to the sodA encoding sequence of E. coli MG1655, a pair of primers was designed and the sodA gene was amplified by PCR techniques. The recombinant plasmid pBV220-sod was constmctied successful and transformed into Lactococcus lactis MG1363 by electroportion. And the expression of sodA gene in Lactococcus lactis was detected by SOD activity assay and SDS-PAGE.

关 键 词：乳酸乳球菌 锰超氧化物歧化酶 PBV220 

分 类 号：R378.992[医药卫生—病原生物学]