人胃癌细胞的黏附过程和整合素β1表达的研究  

Study of the adhering process of human gastric cancer and the expression of intergrinβ1

作　　者：徐远义[1] 黄允宁[2] 马竟先[1] 董俭达[1] 曹相枚[1] 赵琳[1] 柳勇[1] 高宏[1] 

机构地区：[1]宁夏医学院病理教研室,宁夏银川750004 [2]宁夏自治区人民医院消化外科,宁夏银川750004

出　　处：《现代肿瘤医学》2007年第5期595-597,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(30461044);宁夏回族自治区卫生厅科研资助项目(编号W2004010)

摘　　要：目的:观察胃癌细胞黏附过程中的形态学变化,以及在此过程中整合素β1表达的部位和强度,探讨胃癌细胞黏附过程与整合素β1表达之间的关系,为抑制胃癌细胞转移的研究提供依据。方法:借助位相差显微镜观察和激光共聚焦显微镜对活体胃癌MKN1细胞黏附过程中的形态变化和整合素β1的表达部位和程度进行观察。结果:胃癌细胞首先发生形态学变化,细胞伸出伪足与培养盘和其他细胞接触形成稳定状态,在此过程中整合素β1在细胞膜的伪足部分的表达增强,并形成集落团块。结论:人胃癌MKN1细胞在黏附过程中发生形态学变化,并与整合素β1的表达增强有直接关系。Objective： To observe the morphological change of the gastric cancer cells in the adhesive process, and the expression of integrinβ1 in this process, then investigate the relationship of the adhesion process of gastric cancer cells with the expression of integrinβ1. Methods： Laser confocal microscope was used to observe the adhesion process of living MKN1 gastric cancer cell along with the location and expression of intergrinβ1. Results： Gastric cancer cells stabilized to culture plate by the process of forming filopodia, changed into a fiat shape. Integrinβ1 was intensively expressed in the cell membrane and filopodia, and formed clusters in this process. Conclusion： Human gastric cancer MKN1 cell morphological changed in the adhesive process, this process is directly related with the expression of integrinβ1.

关 键 词：胃肿瘤 黏附 整合素Β1 

分 类 号：R730[医药卫生—肿瘤]