端粒酶与癌胚抗原联合测定鉴别良恶性胸腔积液  

Diagnostic value of combining telomerase activity with CEA level in differentiating malignant from nonmalignant pleural effusion

作　　者：陈复辉[1] 陈宏[1] 崔玉华 隋广杰[3] 

机构地区：[1]哈尔滨医科大学附属第二医院呼吸内科,黑龙江哈尔滨150086 [2]哈尔滨市中医院,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第三医院呼吸内科,黑龙江哈尔滨150040

出　　处：《现代肿瘤医学》2007年第5期635-637,共3页Journal of Modern Oncology

基　　金：哈尔滨市科技公关计划(编号:2002AA9CS151-32)

摘　　要：目的:探讨联合检测肿瘤标记物端粒酶、癌胚抗原(CEA)对良恶性胸腔积液的诊断价值。方法:用聚合酶联免疫吸附分析法(PCR-ELISA)检测胸液端粒酶活性,用放射免疫分析法(EIA)测定胸液CEA水平,共检测了26例恶性胸腔积液和32例非恶性胸腔积液。结果:恶性胸腔积液组的胸液端粒酶和CEA的阳性率分别为88%和69%。非恶性胸腔积液的端粒酶和CEA的假阳性率分别为6%和13%。端粒酶活性测定诊断恶性胸腔积液的灵敏度为89%,特异度94%,CEA诊断的灵敏度69%,特异度为88%。结论:检测胸液端粒酶和CEA对鉴别良恶性胸腔积液的诊断均有一定的价值,端粒酶检测恶性胸腔积液的灵敏度和特异度较CEA均高,联合检测综合诊断更能提高诊断准确率。Objective： To investigate the diagnostic value of combining telomerase activity with CEA level in differentiating malignant from nonmalignant pleural effusion . Methods ： Twenty - six specimens of malignant pleural effusion and thirty - two specimens of nonmalignant pleural effusion were examined for telomerase activity and CEA level by PCR - ELISA and EIA, respectively. Results： Positive rates of telomerase activity and CEA were 88% and 69% in group of malignant pleura] effusion . False positive rates of telomerase activity and CEA were 6% and 13% in group of nonmalignant pleural effusion. The diagnostic efficiency of telomerase activity as following： sensitivity 89% , specificity 94%, and CEA as following： sensitivity 69% , specificity 88%. Conclusion： Telomerase activity and CEA have the diagnostic value in differentiating benign from malignant pleural effusion. The results indicate that the detection of telomerase activity in pleural effusions is more sensitive, more accurate than CEA. Diagnostic rates of differentiating benign from malignant will be elevated by detection of combining telomerase activity with CEA .

关 键 词：端粒酶 癌胚抗原 胸腔积液 鉴别诊断 

分 类 号：R730.4[医药卫生—肿瘤]