TSA对人肝癌细胞SMMC-7721的抑制作用及其机制  

The inhibition of TSA to hepatoma cell SMMC-7721 and its mechanism

作　　者：刘维民[1] 闫凯麟[1] 吴智群[1] 

机构地区：[1]第四军医大学唐都医院介入科,陕西西安710038

出　　处：《现代肿瘤医学》2007年第10期1373-1375,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No30572101)

摘　　要：目的:研究去乙酰化转移酶抑制剂TSA对肝癌细胞SMMC-7721的作用及其机理。方法:利用细胞计数,流式细胞仪分析细胞凋亡及细胞周期,Tunel试验研究TSA对肝癌细胞SMMC-7721的作用;利用western研究TSA对肝癌细胞蛋白表达的影响。结果:TSA可明显抑制肝癌细胞SMMC-7721的生长,并可诱导细胞凋亡。可阻滞肝癌细胞SMMC-7721细胞周期于G0/G1期。可增加p53,p21,bax等基因的表达,降低BCL-2的表达。结论:去乙酰化转移酶抑制剂TSA可明显抑制肝癌细胞SMMC-7721的生长并诱导其凋亡,其主要通过调控一些肿瘤相关基因的表达起作用。Objective：To study the role of histone deacetylase inhibitors（TSA） to the hepatoma cell SMMC-7721 and its mechanism.Methods：Cell proliferation assay,Annexin v stain with flow cytometry analysis,cell cycle analysis and Western were used to study the role and mechanisms of TSA to the hepatoma cell SMMC-7721.Results：Histone deacetylase inhibitor（TSA） can inhibit the proliferation of hepatoma cell SMMC-7721 significantly,induce the cell cycle arrest of SMMC-7721 cells,induce the apoptosis of SMMC-7721 cells,increase the expressions of p53,p21,bax and decrease the expression of bcl-2 in hepatoma SMMC-7721 cells.Conclusion：Histone deacetylase inhibitor TSA can inhibit the proliferation of hepatoma cell SMMC-7721 and induce the cell cycle arrest and apoptosis in the hepatoma cells.This maybe related to changes of expression of p53,p21,bax,bcl-2 and other tumor related genes.

关 键 词：肝癌 乙酰化转移酶抑制剂 TSA 肿瘤治疗 

分 类 号：R735.7[医药卫生—肿瘤]