奥沙利铂术前化疗对胃癌细胞增殖与凋亡的作用  

A clinical study of the effect of combined oxaliplatin in preoperative chemotherapy for gastric carcinoma cell apoptosis and proliferating cell nuclear antigen

作　　者：牛子长[1] 王为忠[1] 张洪伟[1] 李孟彬[1] 

机构地区：[1]第四军医大学西京医院胃肠外科,陕西西安710032

出　　处：《现代肿瘤医学》2007年第10期1461-1464,共4页Journal of Modern Oncology

摘　　要：目的:探讨不同的新辅助化疗方案对胃癌细胞凋亡及增殖的影响。寻找一种更为有效的新辅助化疗方案。方法:60例胃癌患者随机分为三组,A组(奥沙利铂+氟尿嘧啶+亚叶酸钙),B组(氟尿嘧啶+亚叶酸钙)及术前未予化疗的对照C组,全身静脉给药,A组奥沙利铂(L-OHP)130mg/m2,VD/2h,d1;氟尿嘧啶(5-FU)400mg/m2,VD快速;600mg/m2,VD缓慢维持22h,d1～d2;亚叶酸钙(CF)300mg,IV,d1～d2。B组氟尿嘧啶(5-FU)400mg/m2,VD快速;600mg/m2,VD缓慢维持22h,d1～d2;亚叶酸钙(CF)300mg,IV,d1～d2。化疗结束后一周内行胃癌根治术。应用原位末端标记法(TUNEL)及免疫组化技术对上述3组患者的胃癌标本进行细胞凋亡及细胞增殖检测。结果:A组胃癌细胞凋亡率为15.75±4.29,B组11.79±3.46,C组6.51±3.24,各组间差异均有统计学意义(P<0.01)。增殖细胞核抗原(PCNA)的阳性率:A组为26.04±9.02,B组为39.49±13.58,C组为57.33±15.89。各组间差异均有统计学意义(P<0.05)。结论:两种新辅助化疗方案均可促进胃癌细胞凋亡并抑制其增殖。含奥沙利铂化疗方案在诱导肿瘤细胞凋亡及抑制肿瘤细胞增殖方面明显优于传统化疗不含奥沙利铂方案。Objective：To investigate the effect of different project of preoperative neoadjuvant chemotherapy for gastric carcinoma cell apoptosis and proliferating cell nuclear antigen apoptosis.To search amore effective preoperative neoadjuvant chemotherapy.Methods：Sixty cases of advanced gastric cancer patients were randomly divided into three groups（A,B and C）,20 cases in each group.GroupA：oxaliplatin＋fluorouracil（Olp＋5Fu）,group B：fluorouracil＋calcium folinate（5-Fu＋CF）,and group C：no preoperative chemotherapy as control,the whole body vein injection administration was performed,A group：oxaliplatin 130mg/m2,VD/2h,d1,fluorouracil 400mg/m2,VD（quickly）,600mg/m2,VD22h,d1~d2,CF 300mg,IV,d1~d2,B group：fluorouracil 400mg /m2,fast vein drop injection;600mg/m2,VD 22h,d1~d2,CF 300mg,IV,d1~d2,gastric carcinoma radical operation was performed within one week after chemotherapy.To use TdT-mediated dUTP nick end labeling（TUNEL） and immunohistochemical methods to detect gastric carcinoma cell apoptosis and proliferating cell nuclear antigen.Result：In A group,apoptosis rate of gastric carcinoma cell was 15.75±4.29,B group was 11.79±3.46,C group was 6.51± 3.244.The difference among all groups all was statistical significante（P〈0.05）.The masculine rate of PCNA in A group was 26.04±9.02,in B group 39.49±13.58,in C group 57.33±15.89（P〈0.05）.Conclusion：Both two preoperative chemotherapy projects can promote gastric carcinoma cell apoptosis and inhibit gastric carcinoma cell propagation. 【Keyword】：oxaliplatin;gastric carcinoma;neoadjuvant chemotherapy;cell apoptosis;proliferating cell nuclear antigen（PCNA）

关 键 词：奥沙利铂 胃癌 新辅助化疗 细胞凋亡 增殖细胞核抗原 

分 类 号：R735.2[医药卫生—肿瘤]