不同代龄SME-1细胞和南方鲶上皮组织同工酶的初步分析  

A PRELIMINARY STUDY ON ISOZYME IN SILURUS SOLDATOVI MERIDINALIS AND SME 1 CELL LINE

作　　者：刘勇[1] 洪锡钧[1] 

机构地区：[1]西南师范大学生命科学系

出　　处：《动物学杂志》1997年第4期16-20,共5页Chinese Journal of Zoology

摘　　要：以不同代龄的ＳＭＥ－１细胞和南方鲶为材料，用聚丙烯酰胺圆盘电泳对ＬＤＨ、ＭＤＨ、α－ＥＳＴ、β－ＥＳＴ和ＳＯＤ同工酶进行了分析。２月龄和１２月龄个体上皮组织中仅α－ＥＳＴ和ＭＤＨ有明显差异，后者酶活力较高。个体和ＳＭＥ－１细胞仅少数谱带一致，多数酶谱明显不同，ＳＭＥ－１细胞中酶谱较少。不同代龄ＳＭＥ－１细胞酶谱有差异，酶活力随着细胞代龄的增加而升高。本文为ＳＭＥ－１细胞的研究及其在细胞生物学、生物工程等方面的应用提供了生化资料。Using polyacrylamide gel disc electrophoresis, the band patterns of LDH, α EST, MDH, β EST and SOD isozyme of SME 1 cell line at different passage and epithelial tissue of S. soldatovi meridinalis have been studied. The results show that α EST and MDH isozyme electrophoretic patterns are significently different in epithelial tissue of two month and twelve month old individuals of S. soldatovi meridinalis. The isozymic activity is higher in the latter. A few isozymic patterns are the same, the major are different between SME 1 cell line and epithelial tissue. The electrophoretic patterns are dissimilar in SME 1 cells at different passage, and the isozymic activity increase when passage become higher, which conform to the metabolic change of the SME 1 cell line.

关 键 词：鲶鱼 SME-1细胞 南方鲶 上皮组织 同功酶 

分 类 号：Q959.468[生物学—动物学]