RNA干扰抑制血管内皮生长因子介导结肠癌细胞凋亡  

Mediating Apoptosis of Colon Cancer by Rna Interference

作　　者：李铁军[1] 王茜[2] 宋建宁[3] 康楷[2] 胡瓒斓[3] 张秉强[1] 向征[1] 张才全[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]重庆医科大学附属第二医院,重庆400010 [3]重庆市第九人民医院,重庆400700

出　　处：《生物技术通报》2007年第6期125-128,共4页Biotechnology Bulletin

摘　　要：目的应用RNA干扰技术抑制血管内皮生长因子(VEGF)表达,并通过体外实验,观察VEGF受抑制后,凋亡情况的变化,为RNA干扰技术的临床应用奠定基础。方法通过Tunel和流式细胞计数的方法,观察VEGF受抑制后,肿瘤细胞凋亡发生率的变化。结果Tunel和流式细胞计数分析显示,当VEGF受抑制后,肿瘤细胞凋亡发生率明显增加。结论Tunel和流式细胞计数分析显示,当VEGF受抑制后,肿瘤细胞凋亡发生率明显增加。Objective：To inhibit VEGF expression by RNA interference in vitro,and observe the apoptosis of colon cancer when VEGF was knocked down,so that RNA interference could be used in clinical therapy.Methods： When the level of VEGF was knocked down,the apoptosis of tumor cells was investgated by Tunel and Flow cytometer.Results：When the level of VEGF was knocked down,Tunel and Flow cytometer showed that the apoptosis of tumor cells were increased. Conclnsion：The inhibition of VEGF could induce the apoptosis of tumor cells in vitro.

关 键 词：RNA干扰 血管内皮生长因子 细胞凋亡 结肠癌细胞 

分 类 号：R735.35[医药卫生—肿瘤]