人信号素基因semaphorin-3F瞬时转染对舌鳞癌细胞增殖作用的影响  

Effect of human semaphoring-3F gene transient transfection on the proliferation of tongue carcinoma cells

作　　者：张嘉佳[1] 吴煜农[1] 叶金海[1] 宋晓萌[1] 

机构地区：[1]南京医科大学口腔医学院口腔颌面外科,210029

出　　处：《中华口腔医学杂志》2007年第12期726-728,共3页Chinese Journal of Stomatology

基　　金：江苏省医学重点人才培养基金(RC2003111)

摘　　要：目的探讨人信号素家族中最具代表性成员信号素3F(semaphorin-3F,SEMA-3F)基因瞬时转染对舌鳞状细胞癌细胞生长的影响。方法将 Tca8113细胞分为4组,即转染组:转染pEGFP-N1-SEMA-3F-cDNA 的 Tca8113细胞;空载体组:转染 pEGFP-N1 质粒的 Tca8113细胞;未转染组:无转染细胞;对照组:仅加转染试剂。构建和纯化 SEMA-3F 的真核表达载体 pEGFP-N1-SEMA-3F,用阳离子脂质体转染试剂介导转染 Tca8113细胞。观察外源目的基因在靶细胞中的表达及细胞的生长情况。结果基因转染后48 h 成功检测到高表达 SEMA-3F 基因。转染后24、48、72、96 h,甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测转染组的各时期平均 A 值,均低于其余3组。转染后48 h,流式细胞仪检测 G_1期细胞比例降低,S 期比例增高。结论 SEMA-3F 基因瞬时转染可使其在 Tca8113细胞中高表达,并使细胞增殖受到抑制。本实验结果将 SEMA-3F 作为目的基因,为治疗舌癌进一步提供了实验依据。Objective To investigate the effect of semaphorin-3F （SEMA-3F） gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells. Methods After construction of a full- length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by LipofectamineTM 2000. The expression of SEMA-3F gene was detected by RT-PCR. The differences of the expression in the transfected cell groups, empty vector groups and untransfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium（MTT） enzymatic labeling technique. Cell cycle were assayed by flow cytometer（ FCM ）. Results The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased. Conclusions SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.

关 键 词：癌 鳞状细胞 基因表达调控 转染 

分 类 号：R739.8[医药卫生—肿瘤]