石蜡包埋口腔白斑组织检测目的基因的方法探讨  

Amplification methods of DNA from paraffin-embedded tissues of oral leukoplakia

作　　者：何园[1] 陈谦明[2] 李秉琦[2] 

机构地区：[1]上海同济大学口腔医学院口腔内科,200072 [2]四川大学华西口腔医学院口腔黏膜病教研室

出　　处：《中华口腔医学杂志》2007年第12期744-746,共3页Chinese Journal of Stomatology

摘　　要：目的探索在石蜡包埋的口腔白斑组织中高效、快捷地检测目的基因的方法。方法采用25例10%福尔马林固定、石蜡包埋的口腔白斑组织,比较显微解剖-巢式 PCR 法、蛋白酶 K 消化-PCR 法与传统酚氯仿抽提-PCR 法3种方法,检测共济失调毛细血管扩张综合征突变基因(ataxiatelangiectasis mutated,ATM)的扩增阳性率。结果 3种方法 PCR 扩增率分别为84%、52%与64%,显微解剖-巢式 PCR 法与蛋白酶 K 消化-PCR 法相比差异有统计学意义(P=0.032)。结论显微解剖-巢式 PCR 方法组织用量少,DNA 检出率高且简便易行,是一种值得推荐的方法。Objective To set up a feasible method for detection of objective genes from paraffinembedded tissues of oral leukoplakia （OLK）. Methods Twenty-five pieces of formalin-fixed, paraffinembedded tissues of OSCC were selected and ATM gene was detected respectively by 3 methods： the microdissection-nested PCR method, proteinase K-PCR method and conventional phenol-chloroform-PCR method. The positivity rates were compared statistically. Results The positivity rates of these 3 methods were 84%, 52% and 64% respectively. Significant difference was found in positivity rate between the microdissection-nested PCR method and the proteinase K-PCR method （ P = 0. 032）. Conclusions The microdissection-nested PCR method merits recommendation because it is more efficient, easy to perform and has the advantage of less sample amount.

关 键 词：石蜡包埋 聚合酶链反应 黏膜白斑病 口腔 

分 类 号：R78[医药卫生—口腔医学]