戊四氮点燃大鼠脑内P-糖蛋白和缺氧诱导因子1α的表达及相关性  

Expression and correlation between P-glycoprotein and Hypoxia inducible factor-1α in brain of rats following pentylenetetrazol-kindled seizure

作　　者：李杏色[1] 庄向阳[2] 柴锡庆[3] 段德义[4] 

机构地区：[1]河北省儿童医院内科 [2]河北石家庄市中心医院神经内科 [3]河北化工医药职业技术学院 [4]首都医科大学神经科学研究所,北京100069

出　　处：《中华神经科杂志》2007年第11期768-770,共3页Chinese Journal of Neurology

基　　金：北京市教育委员会科技发展计划资助项目(KM200410025009)

摘　　要：目的研究戊四氮点燃大鼠脑内 P-糖蛋白(P-gp)和缺氧诱导因子1α(Hif-1α)的表达变化和表达部位,探讨二者之间的关系。方法反复腹腔注射戊四氮(40 mg/kg)制备大鼠点燃模型或注射 PBS 作对照。点燃后不同时间点处死动物。用免疫组织化学染色方法检测 P-gp 和 Hif-1α的表达变化,用双标免疫酶组织化学染色法确定 P-gp 和 Hif-1α的表达部位。结果对照组的 P-gp 和Hif-1α零星分布在毛细血管内皮细胞,点燃组 P-gp 和 Hif-1α分布在海马和皮质神经元的细胞膜,均以点燃后4 h 的表达最高(P-gp:1.09±0.20;Hif-1α:1.12±0.19,F=2.83,P<0.05),且分布方式相似。结论癫癎反复发作可以诱导脑内神经元表达 P-gp 和 Hif-1α,二者可能具有相关性。Objective To explore the correlation between P-glyeopretein （P-gp） and hypoxia inducible factor 1α （Hif-1α） by investigating the expression and location of P-gp and Hif let in the brain of rats after pentylenetetrazol-kindled seizures. Methods Pentylenetetrazol kindling model was induced by intraperitoneal injection of pentylenetetrazol （40 mg/kg, twice daily） , and rats with injection of PBS were used as control. The expression and location of P-gp and Hif-1α were examined by immunohistochemistry and double-label immunostaining. Results In the eontrel group, P-gp and Hif-1α were weakly positive in blood vessels and negative in brain parenehyma. In the kindling group, P-gp and Hif-1α were observed in parenehymal neurons in the cortex and hippocampus, which were co-labelled with NeuN, respectively, but without GFAP. Expression of P-gp and Hif-1α increased to a maximal intensity 4 h after kindling （P-gp： 1.09 ±0. 20; Hif-1α： 1.12 ±0. 19, F =2. 83, P 〈0. 05）. Expression and distribution pattern of P-gp and Hif-1α were similar. Conclusions Recurrent seizures induced by pentylenetetrazol-kindling can induce neuronal expression of P-gp and Hif-1α, suggesting that there probably is eorrelativity between them.

关 键 词：癫痫 戊四唑 P糖蛋白类 核蛋白质类 DNA结合蛋白质类 

分 类 号：R742.1[医药卫生—神经病学与精神病学]